1. Retinal Slices Preparation
- Assemble the chamber (design illustrated in Figure 1). Place two beads of vacuum grease, spaced ~8-10 mm apart, across the recording chamber to form a channel for superfusate and in which to embed the retinal slices. Add a second bead of grease a few millimeters farther out beyond each of these two beads of grease to act as a levee and limit spillover. Place a small triangular piece of KimWipe at the end of the chamber to ensure fluid contact with the reference electrode.
- Press a piece of nitrocellulose membrane (~ 5 x 10 mm; 0.8 μm pores) flat against the glass microscope slide into two small beads of vacuum grease. Avoid putting grease directly beneath the center of the nitrocellulose membrane, as this can prevent the retina from adhering.
- To prepare the tissue slicer, break a double edge razor blade into 4 pieces and attach one to the slicing arm. Cut a thin slice of nitrocellulose membrane to ensure that the cutting edge of the razor blade lays flat against the recording chamber and therefore cuts cleanly through the nitrocellulose membrane.
- Keep a small beaker of amphibian saline solution (Table 1) on ice at the dissection station.
- Euthanize salamander by decapitation. Hemisect the head sagitally and pith through the spinal cord. Place half of the head on a piece of cotton moistened with amphibian saline atop a linoleum block. The other half of the head can be wrapped with a moist paper towel and stored at 4 °C for use later in the day.
- Enucleate the eye. Using small Vannas scissors, cut the skin connecting the eye to the surrounding orbit. After freeing the front of the eye from the surrounding orbital tissue, pull the eye forward and slide the scissors under the eye to cut through the eye muscles and optic nerve, freeing the eye from the orbit.
- Place the enucleated eye on a bed of cotton on the linoleum block. Discard the half head. Trim excess orbital fat from the back of the eye.
- Make a small incision in the center of cornea with a sharp surgical blade. Remove the cornea by sliding fine Vannas scissors into the incision and extending the cut radially out toward the ora serrata. Cut circumferentially around the ora serrata by rotating the linoleum block or the cotton between cuts.
- After cutting all the way around the eye, remove the cornea and lens by pulling them out of the side of the eyecup. Move the resulting eyecup onto a hard surface of the linoleum block moistened with amphibian saline solution. Cut it into thirds with a sharp razor blade, using a fine sawing motion to ensure that you have cut all the way through the sclera.
- Place one or two pieces of eyecup on the nitrocellulose membrane with the retinal surface facing down. Submerge the remaining pieces with additional saline and place them in the refrigerator at ~4 °C.
- Gently press the piece of eyecup against the nitrocellulose membrane with fine forceps. Submerge the nitrocellulose membrane and eyecup piece with several drops of cold amphibian saline and blot at the edges with KimWipe to help the retina to adhere. Once again, submerge the eyecup and nitrocellulose membrane with several drops of cold amphibian saline solution and peel away the sclera/choroid/retinal pigment epithelium to isolate the retina (which can appear pink due to the presence of unbleached rhodopsin). If necessary, cut the optic nerve to free the retina.
- If the retina has not adhered tightly, drain away the saline with a KimWipe to pull the retina more firmly down onto the nitrocellulose membrane. Replace the saline. Repeat, if necessary.
- Fill the chamber with cold amphibian saline and transfer it to the stage of the tissue slicer. Slice the retina and nitrocellulose membrane into thin strips, working from one end to the other by turning the vernier micrometer in 125 μm increments. Press the razor blade gently but firmly through the retina and nitrocellulose membrane.
- Transfer the retinal slices by moving strips of nitrocellulose membrane to the main channel of the recording chamber. Lift a strip of membrane free and then hold it in place while moving the chamber beneath it, being sure to keep the slices submerged. Embed the edges of the nitrocellulose membrane in the strips of vacuum grease, rotating them 90 degrees to view the retinal layers.
- Press the nitrocellulose membrane flat against the glass surface. Even if there is not retina on every piece, place strips of nitrocellulose membrane at regular intervals (~1 mm apart) along the entire length of the perfusion channel to help break up the surface tension and improve fluid flow.
2. Paired Whole-cell Recordings
- After all of the slices have been transferred, move the recording chamber to the stage of an upright, fixed stage microscope and attach the reference electrode lead. Focus on the slices using a long-working distance, water immersion, 40-60X objective. The microscope should be placed on an air table to dampen vibrations and enclosed in a Faraday cage to reduce electrical interference.
- Superfuse the slices continuously at a rate of 1 ml/min with amphibian saline solution bubbled with 100% O2. Connect the suction, making sure that the inflow and outflow are balanced. Outflow can be regulated by rotating the beveled end of the suction needle or by moving the KimWipe at the end of the chamber closer or farther away from the outflow needle. The preparation can be kept at room temperature or cooled with a Peltier device or by simply setting an ice pack on the microscope stage.
- Examine slices under dim or infra-red light and identify a pair of cells – a photoreceptor (rod or cone) and nearby horizontal or bipolar cell – to target for whole-cell recording. Rods can be identified by their large cell bodies and prominent rod-like outer segments (Figure 2A). Cones are smaller than rods and have small tapered outer segments. Bipolar cell and horizontal cell somas are in the outermost row of cell bodies in the inner nuclear layer (INL; Figures 2B and 2C).
- Before preparing slices, use a pipette puller to manufacture micropipettes from borosilicate glass (1.2 mm outer diameter, 0.95 mm inner diameter with a glass filament). The tip of each micropipette should be ~1-2 microns in diameter.
- Using a non-metallic filling needle (e.g. one manufactured from a 1 cc syringe or a MicroFil), fill pipettes with the intracellular solution (Table 1) and attach to the electrode holder.
- Elevate the microscope objective slightly. Position the photoreceptor pipette beneath the objective and then lower it so that the tip is positioned just above the slices. Repeat with the second pipette.
- Adjust any offset in the baseline current level on the amplifier. Check the pipette resistance with a 5-10 mV depolarizing pulse. We typically use pipettes that range from 10-15 MΩ, the result of the long taper of the shaft and low osmolarity of the amphibian pipette solutions. With higher osmolarity mammalian solutions, these same pipettes exhibit resistance values of ~8-12 MΩ. While we have used larger tip diameters with resistance values of 3-4 MΩ in amphibian solutions, the advantages provided by a lower access resistance are offset by a greater difficulty in sealing onto cell membranes and a more rapid rundown of calcium currents and other second messenger-sensitive responses.
- While applying slight positive pressure, position the post-synaptic pipette so that it contacts the horizontal or bipolar cell body. Then position the presynaptic pipette so that it contacts the cell body of a rod or cone photoreceptor. Recordings appear to be more stable when pipette tips contact the inner segment rather than the soma, especially in cones.
- While monitoring the resistance, release the positive pressure on the post-synaptic pipette. Sometimes, the release of positive pressure is sufficient to form a gigaohm seal. If not, apply gentle suction with a 1 ml syringe or by mouth. After the tip resistance has grown to > 100 MΩ, apply a holding potential of -60 mV. After obtaining a gigaohm seal, null out any pipette capacitance transients and repeat the sealing procedure for the photoreceptor pipette, applying a holding potential of -70 mV.
- Rupture the patch by using your mouth or a syringe to apply suction to each cell in turn. Rods, cones, and bipolar cells will typically rupture with gentle suction. Obtaining whole-cell configuration with a horizontal cell may require greater suction (i.e. with a 3 cc syringe) in combination with strong quick voltage pulses delivered with the “zap” feature of the patch clamp amplifier. Rupture of the membrane and establishment of whole-cell configuration will be evident by the appearance of whole-cell capacitance transients.
- Confirm identity of the post-synaptic cell physiologically by applying a light flash and delivering a series of voltage steps from -120 to +40 mV in 20 mV increments (Figures 3 and 4).
- To assess if the pair of cells are synaptically connected, deliver a brief (25-100 msec), 60 mV step depolarization to the photoreceptor (to -10 mV, near the peak of the L-type voltage-gated calcium current) and look for post-synaptic currents in the second order neuron (Figure 5). A strong depolarizing step should evoke a fast, transient inward post-synaptic current in the post-synaptic horizontal or OFF bipolar cell caused by a burst of vesicle release from the cone (Figure 5).
Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
Learning Objectives
Representative traces of light responses from neurons in vertical slices of salamander retina are shown in Figure 3. The cone, horizontal cell, and OFF bipolar cell all display an outward current in response to light onset. The prominent inward current following the light flash in the horizontal and bipolar cell recordings is caused by the increased release of glutamate from photoreceptors as they depolarize at light offset. The ON bipolar cell responds with an inward current at light onset resulting from a sign-inverting metabotropic glutamate receptor signaling cascade and activation of TRPM1 channels9. Horizontal cells and bipolar cells can be distinguished from one another by their I-V relationships (Figure 4). Horizontal cells typically have a linear or inwardly rectifying I-V and low input resistance (<500 MΩ; Figure 4A) while bipolar cells have a high input resistance (0.5-2 GΩ) and outwardly-rectifying I–V (Figure 4B). Figure 5 shows representative results from recordings of a cone-horizontal cell pair (Figure 5A) and cone-OFF bipolar cell pair (Figure 5B). In each, depolarizing the cone to -10 mV from a holding potential of -70 mV evoked a voltage-gated calcium current in the cone and fast inward EPSC in the horizontal or bipolar cell. This strong stimulus is sufficient to empty the readily-releasable pool of ~20 vesicles from each synaptic ribbon, resulting in an EPSC of ~47 pA/ribbon10. The horizontal cell EPSC in Figure 5A was 232 pA, suggesting that it received 5 ribbon contacts from the presynaptic cone. A similar estimate from the 178 pA EPSC in the off bipolar cell (Figure 5B) suggests that it received 4 ribbon contacts from the presynaptic cone.
| Amphibian saline | Presynaptic pipette | Postsynaptic pipette | |
| NaCl | 116 mM | 3.5 mM | 3.5 mM |
| KCl | 2.5 mM | ||
| CaCl2 | 1.8 mM | 1 mM | 1 mM |
| MgCl2 | 0.5 mM | 1 mM | 1 mM |
| HEPES | 10 mM | 10 mM | 10 mM |
| Glucose | 5 mM | ||
| Cs-glutamate* | 40 mM | ||
| Cs-gluconate** | 50 mM | 90 mM | |
| Tetraethylammonium Chloride | 10 mM | 10 mM | |
| ATP-Mg | 9 mM | 9 mM | |
| GTP | 0.5 mM | 0.5 mM | |
| EGTA | 5 mM | 5 mM | |
| pH*** | 7.8 | 7.2 | 7.2 |
| Osmolarity**** | 245 mOsm | 240 mOsm | 240 mOSM |
*Cs-glutamate is made by neutralizing 40 mM L-glutamic acid with 40 mM CsOH in the pipette solution. ** A 1 M stock of Cs-gluconate is made by neutralizing a solution of CsOH with 45-50% D-gluconic acid. ***The pH should be adjusted with NaOH for the extracellular solution and CsOH for the pipette solutions. **** Keeping the osmolarity of the pipette solutions just below that of the extracellular solution prevents cell swelling and enhances longevity of the recording.
Table 1. Components and parameters for the standard intracellular and extracellular solutions used in this protocol.
Figure 1. Recording chamber design. (A-C) Top, side, and cutaway views of the recording chamber showing dimensions. The chamber is machined from a 2 mm thick piece of acrylic. (D) Assembled chamber. Superfusate enters the chamber through a 10 cm length of Teflon tubing (inflow tube; type 24LW). Superfusate is removed by applying mild suction to a beveled 20 gauge metal tube at the other side of the chamber. Adjacent to this output tube is a Ag/AgCl pellet reference electrode. The lead from this reference electrode is connected to the reference input of the headstage. A small triangle of KimWipe is placed over the reference electrode to keep it in contact with the solution and regulate solution flow into the outflow tube. The base of the chamber is formed by placing a glass microscope slide into the recessed edges of the chamber. The slide is held in place with a bead of vacuum grease. Strips of nitrocellulose membrane with retinal slices are embedded in beads of vacuum grease that form a channel for solution to flow. Prior to making slices, a 5 x 10 mm piece of nitrocellulose membrane is affixed to the chamber with two small beads of vacuum grease. A piece of the eyecup is placed vitreal side down on this nitrocellulose membrane and lifted away once the retina adheres. Click here to view larger figure.
Figure 2. Dye-filled cell pairs in vertical slice preparation. A) Images of a rod and synaptically-coupled horizontal cell that were filled with contrasting fluorescent dyes introduced via the patch pipette during simultaneous whole-cell recording. Lucifer yellow (2 mg/ml) was included in the rod pipette solution (yellow) and sulfarhodamine B (1 mg/ml) was included in the horizontal cell pipette solution (purple). Fluorescent images were captured using a spinning disc confocal microscope (Perkin Elmer Ultraview LCI) equipped with a cooled CCD camera (Orca ER) and mounted to the fixed stage microscope (Nikon E600 FN with 60X, 1.0 NA water immersion objective). These images were overlaid on bright field images of the corresponding retinal slices using Adobe Photoshop. B) Images of a cone and synaptically-coupled OFF bipolar cell. The bipolar cell axon terminal ramifies in the outermost (S1) sublamina of the inner plexiform layer near the border with the inner nuclear layer. C) Images of a cone and synaptically-coupled horizontal cell. Note that the cone terminals are considerably larger than the axon terminal of the rod. Although horizontal cells can be identified by the oblong shape of their cell bodies, cell bodies of ON- and OFF-type bipolar cells in the INL cannot be easily distinguished prior to recording. However, one can target displaced cone-driven OFF bipolar cells which have cell bodies in the ONL and can be distinguished from cones by the absence of inner and outer segments. The scale bar is 20 μm.
Figure 3. Light-evoked currents recorded under voltage clamp from four different retinal neurons in response to a bright 500 msec flash of white light. The cone (A), horizontal cell (B), and off bipolar cell (C) all responded to light with an outward current. (D) The on bipolar cell responded to the same light stimulus with an inward current. The baseline noise exhibited by the off bipolar cell (C) reflects a continual release of synaptic vesicles in darkness that diminishes when photoreceptors hyperpolarize in light. Responses from these four cells were obtained in separate recordings using slices prepared under white light. The intensity of the white light stimulus used in these examples produced responses in horizontal and bipolar cells equivalent to 580 nm photon flux of 1 x 105 photons/sec/μm2.
Figure 4. Current-voltage (I-V) relationships of horizontal and off bipolar cells. (A) Top panel, membrane currents evoked by a series of 150 msec voltage steps from -120 to +40 mV applied in 20 mV increments (bottom panel) to a horizontal cell. Horizontal cells typically have a low input resistance and linear or inwardly rectifying I-V relationships. (B) I-V relationship of an off bipolar cell in response to a series of voltage steps. Bipolar cells have a higher input resistance and an outwardly rectifying I-V, due to the activation of voltage-gated potassium currents.
Figure 5. Examples of paired recording data. (A) Recording of the cone voltage-gated calcium current (Cone ICa) in response to a 100 msec step to -10 mV from a holding potential of -70 mV (Cone Vm). Leak and capacitance transients were subtracted using a P/8 leak subtraction protocol. A fast excitatory post-synaptic current (EPSC; HC PSC) was recorded simultaneously from a horizontal cell, showing that these two cells are synaptically coupled. (B) An EPSC was recorded in an off bipolar cell in response to the same stimulus in another cone.
List of Materials
| Name of the reagent/material | Company | Catalogue number | Comments (optional) |
| Tissue slicer | Stoelting | 51425 | |
| Double edge razor blades | Ted Pella, Inc | 121-6 | |
| Nitrocellulose membranes | Millipore | AAWP02500 | Type AAWP 0.8 mm pore |
| Borosilicate glass pipettes | World Precision Instruments | TW120F-4 | 1.2mm OD 0.95 mm ID |
| Ag/AgCl pellet | Warner | E206 | |
| MicroFil | World Precision Instruments | MF34G-5 | 34 ga. Filling needle, 67 mm long |
Lab Prep
One of the central tasks in retinal neuroscience is to understand the circuitry of retinal neurons and how those connections are responsible for shaping the signals transmitted to the brain. Photons are detected in the retina by rod and cone photoreceptors, which convert that energy into an electrical signal, transmitting it to other retinal neurons, where it is processed and communicated to central targets in the brain via the optic nerve. Important early insights into retinal circuitry and visual processing came from the histological studies of Cajal1,2 and, later, from electrophysiological recordings of the spiking activity of retinal ganglion cells – the output cells of the retina3,4.
A detailed understanding of visual processing in the retina requires an understanding of the signaling at each step in the pathway from photoreceptor to retinal ganglion cell. However, many retinal cell types are buried deep in the tissue and therefore relatively inaccessible for electrophysiological recording. This limitation can be overcome by working with vertical slices, in which cells residing within each of the retinal layers are clearly visible and accessible for electrophysiological recording.
Here, we describe a method for making vertical sections of retinas from larval tiger salamanders (Ambystoma tigrinum). While this preparation was originally developed for recordings with sharp microelectrodes5,6, we describe a method for dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells in which we manipulate the photoreceptor’s membrane potential while simultaneously recording post-synaptic responses in horizontal or bipolar cells. The photoreceptors of the tiger salamander are considerably larger than those of mammalian species, making this an ideal preparation in which to undertake this technically challenging experimental approach. These experiments are described with an eye toward probing the signaling properties of the synaptic ribbon – a specialized synaptic structure found in a only a handful of neurons, including rod and cone photoreceptors, that is well suited for maintaining a high rate of tonic neurotransmitter release7,8 – and how it contributes to the unique signaling properties of this first retinal synapse.
One of the central tasks in retinal neuroscience is to understand the circuitry of retinal neurons and how those connections are responsible for shaping the signals transmitted to the brain. Photons are detected in the retina by rod and cone photoreceptors, which convert that energy into an electrical signal, transmitting it to other retinal neurons, where it is processed and communicated to central targets in the brain via the optic nerve. Important early insights into retinal circuitry and visual processing came from the histological studies of Cajal1,2 and, later, from electrophysiological recordings of the spiking activity of retinal ganglion cells – the output cells of the retina3,4.
A detailed understanding of visual processing in the retina requires an understanding of the signaling at each step in the pathway from photoreceptor to retinal ganglion cell. However, many retinal cell types are buried deep in the tissue and therefore relatively inaccessible for electrophysiological recording. This limitation can be overcome by working with vertical slices, in which cells residing within each of the retinal layers are clearly visible and accessible for electrophysiological recording.
Here, we describe a method for making vertical sections of retinas from larval tiger salamanders (Ambystoma tigrinum). While this preparation was originally developed for recordings with sharp microelectrodes5,6, we describe a method for dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells in which we manipulate the photoreceptor’s membrane potential while simultaneously recording post-synaptic responses in horizontal or bipolar cells. The photoreceptors of the tiger salamander are considerably larger than those of mammalian species, making this an ideal preparation in which to undertake this technically challenging experimental approach. These experiments are described with an eye toward probing the signaling properties of the synaptic ribbon – a specialized synaptic structure found in a only a handful of neurons, including rod and cone photoreceptors, that is well suited for maintaining a high rate of tonic neurotransmitter release7,8 – and how it contributes to the unique signaling properties of this first retinal synapse.
Procedure
One of the central tasks in retinal neuroscience is to understand the circuitry of retinal neurons and how those connections are responsible for shaping the signals transmitted to the brain. Photons are detected in the retina by rod and cone photoreceptors, which convert that energy into an electrical signal, transmitting it to other retinal neurons, where it is processed and communicated to central targets in the brain via the optic nerve. Important early insights into retinal circuitry and visual processing came from the histological studies of Cajal1,2 and, later, from electrophysiological recordings of the spiking activity of retinal ganglion cells – the output cells of the retina3,4.
A detailed understanding of visual processing in the retina requires an understanding of the signaling at each step in the pathway from photoreceptor to retinal ganglion cell. However, many retinal cell types are buried deep in the tissue and therefore relatively inaccessible for electrophysiological recording. This limitation can be overcome by working with vertical slices, in which cells residing within each of the retinal layers are clearly visible and accessible for electrophysiological recording.
Here, we describe a method for making vertical sections of retinas from larval tiger salamanders (Ambystoma tigrinum). While this preparation was originally developed for recordings with sharp microelectrodes5,6, we describe a method for dual whole-cell voltage clamp recordings from photoreceptors and second-order horizontal and bipolar cells in which we manipulate the photoreceptor’s membrane potential while simultaneously recording post-synaptic responses in horizontal or bipolar cells. The photoreceptors of the tiger salamander are considerably larger than those of mammalian species, making this an ideal preparation in which to undertake this technically challenging experimental approach. These experiments are described with an eye toward probing the signaling properties of the synaptic ribbon – a specialized synaptic structure found in a only a handful of neurons, including rod and cone photoreceptors, that is well suited for maintaining a high rate of tonic neurotransmitter release7,8 – and how it contributes to the unique signaling properties of this first retinal synapse.