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Bioengineering
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JoVE Journal Bioengineering
Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip
 

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

Article DOI: 10.3791/4290-v • 13:54 min • July 7th, 2012
July 7th, 2012 •
1, 2,3,4, 1, 1, 2,3, 1, 1
1Department of Plant Biology, Carnegie Institution for Science, 2Howard Hughes Medical Institute, 3Departments of Applied Physics and Bioengineering, Stanford University, 4Department of Microsystems Engineering (IMTEK) and Center for Biological Signaling Studies (BIOSS), University of Freiburg

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This article provides a protocol for cultivation of Arabidopsis seedlings in the RootChip, a microfluidic imaging platform that combines automated control of growth conditions with microscopic root monitoring and FRET-based measurement of intracellular metabolite levels.

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Time-lapse Fluorescence Imaging Arabidopsis Root Growth RootChip Root Environment Manipulation Microfluidic Device Nutrient Uptake Sustainable Approaches Crop Yield Microscopic Detail Polydimethylsiloxane (PDMS) Micromechanical Valves Perfusion System Observation Chambers Test Solution Root Biology
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