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Journal / Biology / A Quantitative Fitness Analysis Workflow
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JoVE Journal Biology
A Quantitative Fitness Analysis Workflow
 

A Quantitative Fitness Analysis Workflow

Article DOI: 10.3791/4018-v • 11:40 min • August 13th, 2012
August 13th, 2012 •
*1, *1, 1
1Institute for Cell and Molecular Biosciences, Newcastle University Medical School
* These authors contributed equally

章节

总结概括
Automatic Translation

Quantitative Fitness Analysis (QFA) is a complementary series of experimental and computational methods for estimating microbial culture fitnesses. QFA estimates the effect of genetic mutations, drugs or other applied treatments on microbe growth. Experiments scaling from focussed analysis of single cultures to thousands of parallel cultures can be designed.

Tags

Quantitative Fitness Analysis QFA Fitness Comparison Microbial Cultures Genetic Interaction Drug Screens Growth Curves Quantitative Cell Density Estimates Fitness Measures Genetic Interaction Strengths Drug Sensitivities Substrate Agar Small Molecules Antibiotics Nutrients UV Irradiation Temperature Growth Rate Estimates Spectrophotometric Measurement High Throughput
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