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Isolering og dyrkning af Rotte Embryonale Neurale Celler: En hurtig protokol
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
DOI:

10:51 min

May 24, 2012

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Chapters

  • 00:05Title
  • 01:42Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
  • 03:03PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
  • 04:07Neuronal Dissection and Culture
  • 09:40Results: Isolation and Culture of Rat Embryonic Neural Cells
  • 10:27Conclusion

Summary

自动翻译

Beskriver vi en hurtig metode til isolering og dyrkning hippocampale og corticale neuroner fra gnavere embryoer. Denne protokol tillader os at udføre eksperimenter, hvor næsten rene neuronale kulturer er påkrævet.

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