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适用于培养人类胚胎和诱导多能干细胞的小鼠胚胎成纤维细胞的制备

JoVE 杂志
生物学

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JoVE 杂志生物学

Preparation of Mouse Embryonic Fibroblast Cells Suitable for Culturing Human Embryonic and Induced Pluripotent Stem Cells

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适用于培养人类胚胎和诱导多能干细胞的小鼠胚胎成纤维细胞的制备

DOI:

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09:38 min

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June 21, 2012

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Justyna Jozefczuk, Katharina Drews, James Adjaye

¹Department of Vertebrate Genomics,Max Planck Institute for Molecular Genetics

Chapters

00:05Title
01:55Isolation of Mouse Embryonic Fibroblasts
04:05Inactivation and Plating MEFs (Feeder Cells Preparation)
05:14Conditioned Medium Preparation and Measurement of Activin A using ELISA
07:41Representative Conditioned Medium Preparation Results
09:08Conclusion

Summary

自动翻译

小鼠胚胎成纤维细胞（MEFs）的质量取决于鼠标CF-1如正确的应变。全能性支持MEFs和条件培养基（厘米），从这些应该包含激活素A，小鬼和TGFβ1的最佳浓度为激活素/ Nodal和FGF通路所需的合作保持自我更新和全能性。

Tags

Mouse Embryonic Fibroblast Cells Culturing Human Embryonic Stem Cells Culturing Induced Pluripotent Stem Cells Culture Conditions Gene Expression Pluripotency Feeder Cells Fetal Bovine Serum Knockout Serum Replacement FGF2 Matrigel-coated Plates Conditioned Medium Chemically Defined Conditions Xeno-free Components Mouse Feeder Cells Human Cell Lines Fetal Muscle Cells Skin Cells Amniotic Mesenchymal Cells

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