This content is Free Access.
Chapters
- 00:05Title
- 01:37Anaerobic Buffer
- 02:24Removing Oxygen from the Stopped-flow System
- 03:22Key Solutions
- 04:45Reductive Half-reaction: Monitoring Flavin Reduction
- 07:31Oxidative Half-reaction: Monitoring Flavin Oxidation
- 10:10Stopped Flow: Spectral Forms of Af SidA
- 11:27Conclusion
Vi beskriver bruk av en stoppet-flow instrument for å undersøke både reduktive og oksidativt halv-reaksjoner fra<em> Aspergillus fumigatus</em> Siderophore A (Sida), en Flavin-avhengig monooxygenase. Så viser spektrene tilsvarende artene på den reaksjonen av Sida og vi beregne hastighetskonstanter for dannelse deres.
Tags
Stopped-flow SpectrophotometryFlavin-dependent MonooxygenaseAspergillus Fumigatus Siderophore A (SidA)Hydroxylation Of OrnithineBiosynthesis Of Hydroxamate SiderophoresReductive Half-reactionOxidative Half-reactionFADNADPHC4a-hydroperoxyflavin IntermediateMeasurement Of RatesSpectral FormsStopped-flow InstrumentAnaerobic Glove BoxSingle ModeDouble Mixing Settings
