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Chapters
- 00:05Title
- 01:37Anaerobic Buffer
- 02:24Removing Oxygen from the Stopped-flow System
- 03:22Key Solutions
- 04:45Reductive Half-reaction: Monitoring Flavin Reduction
- 07:31Oxidative Half-reaction: Monitoring Flavin Oxidation
- 10:10Stopped Flow: Spectral Forms of Af SidA
- 11:27Conclusion
Vi beskriver anvendelsen af en stopped-flow instrument at undersøge både reduktive og oxidativ halve reaktioner<em> Aspergillus fumigatus</em> Siderofor A (Sida) et flavin-afhængigt monooxygenase. Vi derefter viser spektre, der svarer til de arter i reaktionen af Sida og vi beregne hastighedskonstanterne for deres dannelse.
Tags
Stopped-flow SpectrophotometryFlavin-dependent MonooxygenaseAspergillus Fumigatus Siderophore A (SidA)Hydroxylation Of OrnithineBiosynthesis Of Hydroxamate SiderophoresReductive Half-reactionOxidative Half-reactionFADNADPHC4a-hydroperoxyflavin IntermediateMeasurement Of RatesSpectral FormsStopped-flow InstrumentAnaerobic Glove BoxSingle ModeDouble Mixing Settings
