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Att avgöra den optimala cytotoxiska aktiviteten hos mänskliga Her2neu specifika CD8 T-celler genom att jämföra Cr51 frisättningsanalysen till xCELLigence System
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System
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Att avgöra den optimala cytotoxiska aktiviteten hos mänskliga Her2neu specifika CD8 T-celler genom att jämföra Cr51 frisättningsanalysen till xCELLigence System

24,592 Views

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11:31 min

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August 08, 2012

DOI:

10.3791/3683-v

DOI:
10.3791/3683-v

•

11:31 min
August 08, 2012

•

5 Views
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1Department of Immunology,College of Medicine, Mayo Clinic

Tags

Cytotoxic ActivityCD8 T CellsCr51 Release AssayXCELLigence SystemT Cell Receptor (TCR)Antigen-specific CD8 T CellsELIspotFlow CytometryCytotoxicity AssayChromium Release Assay (CRA)Gamma RadiationReproducibilityCaspase ReleaseBLT Esterase ActivityCD107 ExpressionImpedance-based AssayRoche XCelligence System

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Erskine, C. L., Henle, A. M., Knutson, K. L. Determining Optimal Cytotoxic Activity of Human Her2neu Specific CD8 T cells by Comparing the Cr51 Release Assay to the xCELLigence System. J. Vis. Exp. (66), e3683, doi:10.3791/3683 (2012).

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