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Kilitli Nükleik Asit Sitometrisi-floresan Yerinde Hibridizasyon (LNA akış FISH): Bakteriyel Küçük RNA Algılama Yöntemi
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January 10, 2012
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Yeni yüksek verimli bir yöntem kilitli nükleik asit probları ve flow sitometri floresan kullanarak tek bir bakteri hücrelerinin algılama ve göreceli küçük RNA ve mRNA ekspresyonu kantitatif açıklanan<em> Yerinde</em> Melezleşme.
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Locked Nucleic AcidFlow CytometryFluorescence In Situ HybridizationFISHNucleic Acid SequencesFlow-FISHLysate-based Nucleic Acid Detection MethodsStatistical AnalysisVariance AnalysisTelomere Length DeterminationCellular IdentificationGene ExpressionViral MultiplicationBacterial Community AnalysisDNA Oligonucleotide ProbesNucleic Acid AnalogsSensitivitySpecificityMelting Temperatures (Tm)Locked Nucleic Acid (LNA) Probes
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Robertson, K. L., Vora, G. J. Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection. J. Vis. Exp. (59), e3655, doi:10.3791/3655 (2012).
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