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再生的成年斑马鱼尾鳍在吗啉代入体内电穿孔
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
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再生的成年斑马鱼尾鳍在吗啉代入体内电穿孔

DOI:
10.3791/3632-v

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07:22 min

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March 29, 2012

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, ,
1Department of Biological Sciences, Center for Zebrafish Research,University of Notre Dame , 2Department of Microbiology, Immunology, and Pathology,Colorado State University , 3Departments of Anatomy and Cell Biology and Ophthalmology,Wayne State University School of Medicine

Chapters

  • 00:05Title
  • 01:19Morpholino Resuspension and Fin Amputation
  • 02:37Morpholino Injection
  • 04:05Electroporation
  • 05:14Analysis
  • 06:17Representative Fin-outgrowth Results
  • 06:55Conclusion

Summary

自动翻译

自动翻译

我们描述了一个方法，有条件击倒目标蛋白的表达，在成年斑马鱼的鳍再生。这项技术涉及微注射和反义寡核苷酸进入鳍组织，它允许测试鳍再生，伤口愈合，芽基形成和再生产物，包括在不同阶段的蛋白质的作用吗啉代电穿孔。

Tags

In Vivo ElectroporationMorpholinosRegenerating Adult Zebrafish Tail FinUrodelesTeleost FishZebrafish ModelSpontaneous RegenerationHeart RegenerationRetina RegenerationSpinal Cord RegenerationOptic Nerve RegenerationSensory Hair Cell RegenerationFin Regeneration StagesWound HealingBlastema FormationFin OutgrowthEpithelium ProliferationCell Cycle Re-entryProliferative Blastema FormationRe-differentiation Of CellsGene Expression During Fin Regeneration

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