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高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
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高效的推导人类心脏的前体和从多能干的小分子诱导人类胚胎干细胞的心肌细胞

DOI:
10.3791/3274-v

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10:46 min

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November 03, 2011

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, , , , ,
1San Diego Regenerative Medicine Institute, 2Xcelthera, 3Department of Neurosurgery,Harvard Medical School, 4Division of SCI Research,VA Boston Healthcare System, 5Program in Stem Cell & Regenerative Biology,Sanford-Burnham Medical Research Institute, 6La Jolla IVF

Chapters

  • 00:05Title
  • 01:22Media Preparation and Plate Coating
  • 02:30Passing and Seeding Undifferentiated Human ES Cells
  • 04:27Cardiac Induction in Adhesive Culture with Nicotinamide
  • 05:37Differentiation in Suspension Culture and Maturation to Beating Cardiomyocyte Phenotype after Attachment
  • 08:05Direct Cardiomyocyte Differentiation of Feeder- and Serum-Free Pluripotent Humand ES Cells with Nicotinamide Induction
  • 10:23Conclusion

Summary

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自动翻译

我们已经建立了诱导多能干人类胚胎干细胞与小分子物质，使推导一个人类心脏的祖细胞和心血管修复功能心肌的大量供应定义的条件下保持直接从cardioblasts协议。

Tags

Human Cardiac PrecursorsCardiomyocytesPluripotent Human Embryonic Stem CellsSmall Molecule InductionRegenerating Human MyocardiumCell-based TransplantationStem/progenitor CellsBone MarrowCord BloodContractile Heart Muscle CellsAdult Stem Cell TherapyGenetically Stable Human Embryonic Stem CellsUnlimited Expansion AbilityUnrestricted PlasticityLarge Supplies Of Human Somatic CellsCardiovascular DiseaseDonor OrgansHESC-based Therapies

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