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An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Chapters
- 00:05Title
- 02:00Preparation of Whole Cell Template
- 03:29PCR Set-up
- 06:45Gel Electrophoresis
- 09:20Multiplexing PCR Results: Identifying Specific O and H Alleles Associated with S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
- 11:51Conclusion
We describe a multiplex PCR for the rapid detection of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. Specific Salmonella serovars can be identified by targeting a multiplex PCR to genes and sequences unique to the O-antigen biosynthesis cluster and flagellin of a given serovar. Serovar is assigned then to a Salmonella isolate based on the appearance of specific, size amplicons (PCR product) corresponding to the target allele.
Tags
Allelotyping PCRIdentifying Salmonella Enterica SerovarsEnteritidisHadarHeidelbergTyphimuriumCommercial PCR TestsSalmonella Target GenesSalmonella SerovarsPublic Health SignificanceS. Enterica Subspecies IIIa ArizonaS. Enterica Subspecies I Serovar EnteritidisSalmonellosisTable EggsLipopolysaccharide (LPS)Flagellin (H1 And H2 Antigens)Antigenic DifferencesGenetic DifferencesAllelotyping Multiplex PCRPoultry-associated DiseasesUS
