需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Non-Laser Capture Microscopy Ansatz für die Mikrodissektion von Discrete-Maus Hirnregionen für Gesamt-RNA Isolierung und Downstream Next-Generation Sequencing and Gene Expression Profiling
Chapters
- 00:05Title
- 01:40Sampling Setup and Preparation
- 04:30Brain Removal and Coronal Sectioning
- 06:05Brain Region Microdissection and Tissue Archival
- 07:44Total RNA Extraction
- 09:01Representative RNA Isolation Results
- 09:38Conclusion
RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.
Tags
Non-laser Capture MicroscopyMicrodissectionMouse Brain RegionsTotal RNA IsolationNext-generation SequencingGene Expression ProfilingTechnological PlatformsNext-generation SequencingMicroarrayQRT-PCRHigh-resolution RNA SequencingRNA-SeqTranscript LevelsAlternative SplicingMicro RNAsWhole Transcriptome ProfilingPhenotypically Distinct GroupsMolecular MechanismsPhysiological StatesDisease ProgressionNext-generation Sequencing IntegrationGene Expression StudiesMouse ModelsBrain Nuclei PrecisionLaser Capture Microscopy (LCM)Sample Excision
