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通过注射和电穿孔的斑马鱼Otocyst的MIF Morpholinos直接运抵影响内耳发展
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
DOI:

10:40 min

January 07, 2011

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Chapters

  • 00:05Title
  • 01:22Introduction
  • 02:45Making Electrodes and Set Up of Electroporation Station
  • 04:09Microinjection of Morpholinos into the Zebrafish Otic Vesicle
  • 06:52Electroporation Procedure
  • 08:13Representative Ear Injection Results
  • 10:21Conclusion

Summary

自动翻译

在24hpf的斑马鱼otocyst morpholinos直接传递到一个方法来已经研制成功。管腔奥迪的囊泡和电的影响渗透到使用morpholinos显微注射,我们能够绕过morpholinos对大脑的影响,并获得具体到内耳的影响。

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