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Espansione ex vivo di tumore cellule T reattive per mezzo di 1/Ionomycin Bryostatin e il Comune catena gamma Citochine Formulazione
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Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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Espansione ex vivo di tumore cellule T reattive per mezzo di 1/Ionomycin Bryostatin e il Comune catena gamma Citochine Formulazione

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07:20 min

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January 14, 2011

DOI:

10.3791/2381-v

DOI:
10.3791/2381-v

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07:20 min
January 14, 2011

•

1 Views
, , , ,
1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Summary

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Un protocollo efficiente per la<em> Ex vivo</emEspansione> di tumore cellule T reattive da tumore-drenante linfonodi o altri tessuti linfatici secondari del tumore-cuscinetto ospita è descritto. Questo protocollo si espande selettivamente tumore-specifiche cellule T per l'uso in immunoterapia adottiva di cancro al seno.

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Kmieciak, M., Toor, A., Graham, L., Bear, H. D., Manjili, M. H. Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation. J. Vis. Exp. (47), e2381, doi:10.3791/2381 (2011).

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