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Ex vivo Uitbreiding van Tumor-reactieve T-cellen door middel van Bryostatin 1/Ionomycin en de Gemeenschappelijke Gamma Chain Cytokines Formulering
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Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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Ex vivo Uitbreiding van Tumor-reactieve T-cellen door middel van Bryostatin 1/Ionomycin en de Gemeenschappelijke Gamma Chain Cytokines Formulering

14,588 Views

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07:20 min

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January 14, 2011

DOI:

10.3791/2381-v

DOI:
10.3791/2381-v

•

07:20 min
January 14, 2011

•

1 Views
, , , ,
1Department of Microbiology & Immunology,Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine,Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery,Virginia Commonwealth University- Massey Cancer Center

Summary

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Een efficiënte protocol voor de<em> Ex vivo</em> Uitbreiding van de tumor-reactieve T-cellen van tumor-drainerende lymfeklieren of andere secundaire lymfoïde weefsels van tumor-dragende hosts wordt beschreven. Dit protocol breidt selectief tumor-specifieke T-cellen voor gebruik in de adoptieve immunotherapie van borstkanker.

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Kmieciak, M., Toor, A., Graham, L., Bear, H. D., Manjili, M. H. Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation. J. Vis. Exp. (47), e2381, doi:10.3791/2381 (2011).

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