Diffuse Correlation Spectroscopy to Assess Cerebral Blood Flow in a Mouse Model

Allow the mouse to recover in the supine position on a 37-degree Celsius warming pad. After a two-minute period of stabilization, gently rest the DCS sensor over the right hemisphere of the mouse's skull, such that the top edge of the optical sensor, lines up with the back of the eye, and the side of the sensor lines up along the midline. Cup a hand over the sensor to shield it from room light, and acquire five seconds of data. Then, reposition the sensor over the left hemisphere, and acquire five seconds of left hemisphere data.