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Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method

Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method

DOI:
10.3791/2111-v

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09:19 min

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September 06, 2010

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, ,
1Department of Neurosurgery,Cedars Sinai Medical Center, UCLA, 2Basic Medicine School,Fourth Military Medical University, 3Department of Neurology,David Geffen School of Medicine, UCLA, 4Aerospace Medicine School,Fourth Military Medical Univeristy

Chapters

  • 00:00Title
  • 01:03Introduction
  • 01:34Establishing a Glial Cell Culture
  • 04:29Isolation, Purification, and Cuturing of Dorsal Root Ganglion Neurons (DRGs) and Coculturing of DRGs with Glial Cells
  • 07:29Representative Cultures of Glial Cells, DRG Neurons, and Cocultures of Both Cell Types
  • 08:36Conclusion

Summary

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In this protocol, we described a new method to study the influence of glial cell heterogeneity on axon growth with an in vitro co-culture system. Rat cortical glial cells were cultured to confluence and cocultured with highly purified rat dorsal root ganglia neurons. Different glial cell influence on neurons adhesion and axon growth was compared directly in the same culture. This method provides a new way to directly study the glial cell heterogeneity influence on neuron adhesion and axon growth.

Tags

Glial Cell HeterogeneityAxon GrowthCoculture MethodCentral Nervous SystemRegenerative CapacityInhibitory MyelinAstrocytesNeuron BehaviorDorsal Root Ganglia Neurons

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