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Genoma Analisi mediante ChIP per identificare Isoform obiettivi specifici per Gene
JoVE 杂志
生物学
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JoVE 杂志 生物学
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
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Genoma Analisi mediante ChIP per identificare Isoform obiettivi specifici per Gene

14,209 Views

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11:19 min

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July 07, 2010

DOI:

10.3791/2101-v

DOI:
10.3791/2101-v

•

11:19 min
July 07, 2010

•

1 Views
, , , , , ,
1Department of Biochemistry and Molecular Genetics,University of Illinois Chicago – UIC, 2Research Unit on Biomedical Informatics,Universitat Pompeu Fabra, 3Genome Technology Core,Whitehead Institute for Biomedical Research

Summary

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Qui stiamo presentando un immunoprecipitazione della cromatina (ChIP) procedura per la genoma analisi posizione di isoforme di proteine ​​che si differenziano in un istone-dominio di legame. Lo stiamo applicando a ChIP-Seq analisi per identificare gli obiettivi del demetilasi KDM5A/JARID1A/RBP2 istoni.

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Cite this Article

Beshiri, M. L., Islam, A., DeWaal, D. C., Richter, W. F., Love, J., Lopez-Bigas, N., Benevolenskaya, E. V. Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets. J. Vis. Exp. (41), e2101, doi:10.3791/2101 (2010).

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