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Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants
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神经科学
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JoVE 杂志 神经科学
Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants

Targeting of Deep Brain Structures with Microinjections for Delivery of Drugs, Viral Vectors, or Cell Transplants

DOI:
10.3791/2082-v

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07:00 min

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December 01, 2010

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, ,
1 Neuroscience Lab/ Fac. Psicologia,University of Colima, 2Department of Neurosurgery,Johns Hopkins University

Chapters

  • 00:05Title
  • 00:55Introduction
  • 01:18Constructing Glass Needles
  • 02:36Using the Needles for Microinjections into the Rodent Brain
  • 06:06Representative Microinjection Results
  • 06:36Conclusion

Summary

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In this article, we show a method to make glass capillary needles with a 50-μm lumen. This technique significantly reduces the brain damage, minimizes passive diffusion of drugs and allows a precise targeting into the rodent brain.

Tags

Deep Brain StructuresMicroinjectionsDrug DeliveryViral VectorsCell TransplantsBrain ParenchymaInjecting NeedleBrain LesionMouse BrainGlass Capillary NeedlesBrain DamagePrecise TargetingRodent BrainSmall VolumesBleeding RisksPassive DiffusionInjection TechniquesCollateral Damage

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