This content is Free Access.
Chapters
- 00:00Title
- 00:59Introduction
- 01:25Experimental Set-up
- 04:33Isolated Heart Preparation
- 07:41Assessment of Cardiac Function and Energetics Using 31P NMR Spectroscopy
- 09:43Representative Results of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
- 11:09Conclusion
Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.
Tags
Cardiac FunctionEnergeticsIsolated Mouse Hearts31P NMR SpectroscopyBioengineered Mouse ModelsCardiovascular DiseaseMolecular AlterationsSignaling PathwayPhysiologyBiochemical AssessmentMetabolitesIntact Beating HeartIsolated Heart PerfusionsNuclear Magnetic Resonance (NMR) SpectroscopyLeft Ventricular FunctionLangendorff-mode PerfusionsCardiac Energetics31P Magnetic Resonance SpectroscopyPhosphocreatine And ATP LevelsPhysiologic StressorsPathologic StressorsIschemia/reperfusionHigh Workload Challenge ProtocolsAortic BandingCardiac Pathology
