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Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants

JoVE 杂志
免疫与感染

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JoVE 杂志免疫与感染

Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants

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Assembling Ha-CoV-2 Particles by Cotransfection of HEK293T Cells for Quantification of SARS-CoV-2 Variants

DOI:

10.3791/201201-v

•

01:40 min

•

September 08, 2023

•

Linda Chilin, Brian Hetrick, Yuntao Wu

¹Center for Infectious Disease Research, School of Systems Biology,George Mason University

Tags

HEK293T Cells DMEM FBS Penicillin Streptomycin Trypan Blue Cell Counting Cell Culture Cotransfection Viral Particle Assembly SARS-CoV-2 Variants Ha-CoV-2 Pseudovirus Centrifugation Filtration Storage

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