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An In Situ Immunostaining Protocol for Parkinson's Disease Study
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Mitochondrial Morphology Analysis by Immunohistochemistry on Paraffin-Embedded Brain Slices from α-Synuclein Pre-Formed Fibrils (PFF)-Injected Mice
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Mitochondrial Morphology Analysis by Immunohistochemistry on Paraffin-Embedded Brain Slices from α-Synuclein Pre-Formed Fibrils (PFF)-Injected Mice
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Mitochondrial Morphology Analysis by Immunohistochemistry on Paraffin-Embedded Brain Slices from α-Synuclein Pre-Formed Fibrils (PFF)-Injected Mice

An In Situ Immunostaining Protocol for Parkinson's Disease Study

DOI:
10.3791/201001-v

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04:21 min

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June 23, 2023

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, , , , , ,
1Achucarro Basque Center for Neuroscience, 2Department of Neuroscience,University of the Basque Country (UPV/EHU), 3Ikerbasque – Basque Foundation for Science, 4Oxford Parkinson’s Disease Centre and Department of Physiology, Anatomy and Genetics,University of Oxford

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Keywords: Mitochondrial MorphologyImmunohistochemistryParaffin-embeddedBrain Slicesα-synucleinPre-formed Fibrils (PFF)C57 Black Six MiceCitrate BufferTBS-TBlockingPrimary AntibodySecondary AntibodyDAPIMounting Reagent

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