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Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
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生物学
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JoVE 杂志 生物学
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes

Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes

DOI:
10.3791/195-v

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15:27 min

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May 28, 2007

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, ,
1Department of Environmental Science and Engineering,California Institute of Technology – Caltech

Chapters

  • 00:14Part I – Extraction of DNA from termite gut microbes
  • 00:19Introduction
  • 01:34Critical reagents
  • 03:08Raising termites in the laboratory
  • 04:39Isolating the termite gut track
  • 06:00Isolating DNA
  • 10:27Part II Making wet mount and visualizing hindgut microbes
  • 14:24Conclusion

Summary

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自动翻译

This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.

Tags

DNA ExtractionGut MicrobesTermiteZootermopsis AngusticollisVisualizationLignocellulose DegradationMicrobial PopulationsBacteriaMethanogenic ArchaeaEukaryotic ProtozoaMolecular TechniquesNucleic AcidsExtraction And Purification

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