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1Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California, San Francisco – UCSF, 2Department of Pathology and Cell Biology,College of Physicians and Surgeons, Columbia University, 3Department of Neuroscience and Neurology,College of Physicians and Surgeons, Columbia University, 4Department of Developmental and Regenerative Biology,Nagoya City University Graduate School of Medical Sciences, 5Center for Motor Neuron Biology and Disease,College of Physicians and Surgeons, Columbia University
Chapters
- 00:00Title
- 00:07Introduction
- 01:02Wholemount Dissection
- 04:14Ependymal Flow Analysis Using Fluorescent Microbeads
- 06:41Fixing and Immunostaining Wholemounts
- 10:58Representative Images and Ependymal Flow Data
- 12:20Conclusion
Den laterala ventrikeln väggarna innehåller den största germinalcenter regionen i den vuxna däggdjur hjärnan. Traditionellt har studier på neurogenes i denna region förlitat sig på klassiska sektionering tekniker för histologisk analys. Här presenterar vi en alternativ metod, den wholemount teknik, som ger en omfattande, sv-ansikte bakgrund av detta embryon regionen.
