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1Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California, San Francisco – UCSF, 2Department of Pathology and Cell Biology,College of Physicians and Surgeons, Columbia University, 3Department of Neuroscience and Neurology,College of Physicians and Surgeons, Columbia University, 4Department of Developmental and Regenerative Biology,Nagoya City University Graduate School of Medical Sciences, 5Center for Motor Neuron Biology and Disease,College of Physicians and Surgeons, Columbia University
Chapters
- 00:00Title
- 00:07Introduction
- 01:02Wholemount Dissection
- 04:14Ependymal Flow Analysis Using Fluorescent Microbeads
- 06:41Fixing and Immunostaining Wholemounts
- 10:58Representative Images and Ependymal Flow Data
- 12:20Conclusion
As paredes ventrículo lateral contêm a maior região germinal no cérebro adulto de mamíferos. Tradicionalmente, os estudos sobre a neurogênese na região têm contado com técnicas clássicas de corte para análise histológica. Aqui nós apresentamos uma abordagem alternativa, a técnica wholemount, que fornece uma visão completa e en face desta região germinal.
