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脑室下区恩的脸：Wholemount染色和室管膜流量
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May 06, 2010
DOI:
1Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California, San Francisco – UCSF, 2Department of Pathology and Cell Biology,College of Physicians and Surgeons, Columbia University, 3Department of Neuroscience and Neurology,College of Physicians and Surgeons, Columbia University, 4Department of Developmental and Regenerative Biology,Nagoya City University Graduate School of Medical Sciences, 5Center for Motor Neuron Biology and Disease,College of Physicians and Surgeons, Columbia University
Summary
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侧脑室壁中含有生发在成年哺乳动物大脑中最大的地区。传统上，对神经发生在这一地区的研究都依赖于经典切片技术进行组织学分析。在这里，我们提出了一种替代方法，wholemount技术，它提供了一个全面的，面对这生发地区。
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Mirzadeh, Z., Doetsch, F., Sawamoto, K., Wichterle, H., Alvarez-Buylla, A. The Subventricular Zone En-face: Wholemount Staining and Ependymal Flow. J. Vis. Exp. (39), e1938, doi:10.3791/1938 (2010).
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