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1Department of Neurosurgery, The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research,University of California, San Francisco – UCSF, 2Department of Pathology and Cell Biology,College of Physicians and Surgeons, Columbia University, 3Department of Neuroscience and Neurology,College of Physicians and Surgeons, Columbia University, 4Department of Developmental and Regenerative Biology,Nagoya City University Graduate School of Medical Sciences, 5Center for Motor Neuron Biology and Disease,College of Physicians and Surgeons, Columbia University
Chapters
- 00:00Title
- 00:07Introduction
- 01:02Wholemount Dissection
- 04:14Ependymal Flow Analysis Using Fluorescent Microbeads
- 06:41Fixing and Immunostaining Wholemounts
- 10:58Representative Images and Ependymal Flow Data
- 12:20Conclusion
The lateral ventricle walls contain the largest germinal region in the adult mammalian brain. Traditionally, studies on neurogenesis in this region have relied on classical sectioning techniques for histological analysis. Here we present an alternative approach, the wholemount technique, which provides a comprehensive, en-face view of this germinal region.
