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Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

DOI:
10.3791/1662-v

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12:55 min

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January 07, 2010

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, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc.

Chapters

  • 00:00Title
  • 00:44Introduction
  • 02:07Preparing the Mouse Embryonic Fibroblasts
  • 04:00The 96-well Electroporation Plates
  • 05:20Electroporating the MEFs in 96-well Plates
  • 07:49Electroporating the MEFs in Cuvettes
  • 10:11Epifluorescence Microscopy Images of Transfected Cells
  • 12:26Conclusion

Summary

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This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.

Tags

Gene Pulser MXcell Electroporation SystemTransfectPrimary CellsHigh EfficiencyPlasmid DNASiRNAElectroporation BufferBio-RadNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsElectroporation ConditionsOptimization96-well Electroporation PlatesElectroporation CuvettesElectroporation EfficiencySuccess Or Failure Of Electroporation Experiments

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