Non-radioactive <em>in situ</em> Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Anna Karlgren; Jenny Carlsson; Niclas Gyllenstrand; Ulf Lagercrantz; Jens F. Sundstr&#246;m

doi:10.3791/1205

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生物学

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Non radioactifs In situ Applicable pour l'épinette de Norvège et d'un éventail d'espèces végétales

JoVE 杂志
生物学
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JoVE 杂志生物学

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

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Non radioactifs In situ Applicable pour l'épinette de Norvège et d'un éventail d'espèces végétales

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11:56 min

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April 17, 2009

DOI:

10.3791/1205-v

DOI:

10.3791/1205-v

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11:56 min

April 17, 2009

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Anna Karlgren, Jenny Carlsson, Niclas Gyllenstrand, Ulf Lagercrantz, Jens F. Sundström

¹Department of Evolutionary Functional Genomics, Evolutionary Biology Center,Uppsala University, ²Department of Plant Biology and Forest Genetics, Uppsala BioCenter,Swedish University of Agricultural Sciences

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Non-radioactive In Situ Hybridization Protocol Norway Spruce Plant Species Expression Analysis Technologies Expression Profiles In Situ Hybridization MRNA Expression Laborious Time-consuming Optimization Woody Species Conifer Picea Abies Modified DIG In Situ Hybridization Protocol RNase Treatment Proteinase K Concentration Homologous Genes Male Reproductive Organs Gymnosperm Angiosperm Species Arabidopsis Thaliana Brassica Napus Floral Organs Microsporophylls

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Karlgren, A., Carlsson, J., Gyllenstrand, N., Lagercrantz, U., Sundström, J. F. Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species. J. Vis. Exp. (26), e1205, doi:10.3791/1205 (2009).

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