Evaluating the Anti-Tumor Efficacy of a Bacterial Immunomodulatory Protein in a Murine Model
Abstract
Source: Codolo, G. et al. Evaluation of the Efficacy of the H. pylori Protein HP-NAP as a Therapeutic Tool for Treatment of Bladder Cancer in an Orthotopic Murine Model. J. Vis. Exp. (2015)
This video demonstrates a technique to assess the anti-tumor activity of Helicobacter pylori neutrophil-activating protein, or HP-NAP, in a mouse bladder cancer model. HP-NAP interacts with tumor-cell neutrophils to produce pro-inflammatory cytokines, inducing the differentiation of naïve T cells into type 1 T helper (Th1) and type 1 cytotoxic (Tc1) T cell phenotypes. The Th1 and Tc1 cells secrete interferon-gamma, inhibiting tumor angiogenesis and promoting cytotoxic T cell-mediated tumor destruction, reducing tumor volume.
Protocol
Disclosures
The authors have nothing to disclose.
Materials
| C57BL/6J female mice | Harlan Italy (Udine, Italy) | ||
| MB49 Cells | Obtained from Prof. O'Donnel, University of Iowa Carver College of Medicine, Iowa, USA | ||
| RPMI | Sigma-Aldrich (St. Louis, Missouri, USA) | R8758 | |
| FBS | Sigma-Aldrich | F7524 | |
| PBS | Sigma-Aldrich | D1408 | 10X, to be diluted in apyrogenic water |
| Flask | Becton Dickinson (Franklin Lakes, New Jersey, USA) | 353135 | |
| Syringe 1ml | Becton Dickinson | 301358 | |
| Trypsin | Life Technologies (Waltham, Massachusetts, USA) | ||
| Gentamycin | Life Technologies | 15710-049 | |
| Xilor | Bio 98 s.r.l. (Milano, Italy) | 2% Xylazin | |
| Zoletil | Virbac (Carros, France) | 3.59713E+11 | 5% Zolazepam + 5% Tiletamine |
| 24G Catheter | Terumo (Rome, Italy) | SR+DM2419PX | |
| HCl | Carlo Erba Reagents (Milano, Italy) | 403871 | Liquid |
| NaOH | JT Baker (Center Valley, Pennsylvania, USA) | 10095011 | Powder |
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