Antibodies |
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|
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Biotinylated goat anti-human Igs |
Thermo Fisher Scientific, Waltham, MA, USA |
Ref. #: 31782 |
|
Biotinylated goat anti-mouse IgM + IgG |
Thermo Fisher Scientific |
Ref. #: 31807 |
|
Equipment |
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|
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Robotic Arrayer sciFLEXARRAYER S5 |
Scienion AG, Berlin, Germany |
http://www.scienion.com/products/sciflexarrayer/ |
|
Stain Tray (slide incubation chamber) |
Simport, Beloeil, QC, Canada |
Ref. #: M920-2 |
|
Centrifuge |
Eppendorf, Hamburg, Germany |
Ref. #: 5810 R |
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Pipettes |
Gilson, Middleton, WI, USA |
http://www.gilson.com/en/Pipette/ |
|
Slide Scanner |
PerkinElmer, Waltham, MA, USA |
ScanArray GX Plus |
|
Shaking incubator |
Cole-Parmer, Staffordshire, UK |
Ref. #: SI50 |
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Biological samples |
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|
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BALB/c mice sera |
This paper |
N/ A |
|
Complex Immunoglobulin Preparation (CIP) |
Immuno-Gem, Moscow, Russia |
http://www.biomedservice.ru/price/goods/1/17531 |
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Chemicals, Reagents and Glycans |
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|
|
Glycan library |
Institute of Bioorganic Chemistry (IBCh), Moscow, Russia |
N/ A |
|
Bovine serum albumin (BSA) |
Sigma-Aldrich, St. Louis, MO, |
Ref. #: A9418 |
|
Ethanolamine |
Sigma-Aldrich |
Ref. #: 411000 |
|
Tween-20 |
Merck Chemicals & Life Science S.A., Madrid, Spain |
Ref. #: 655204 |
|
Phospahte-buffered saline (PBS) |
VWR International Eurolab S.L, Barcelona, Spain |
Ref. #: E404 |
|
Sodium azide |
Sigma-Aldrich |
Ref. #: S2002 |
|
Streptavidin Alexa Fluor 555 conjugate |
Thermo Fisher Scientific |
Ref. #: S21381 |
|
Streptavidin Cy5 conjugate |
GE Healthcare, Little Chalfont, Buckinghamshire, UK |
Ref. #: PA45001 |
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Materials |
|
|
|
N-hydroxysuccinimide-derivatized glass slides H |
Schott-Nexterion, Jena, Germany |
Ref. #: 1070936 |
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Whatman filter paper |
Sigma-Aldrich |
Ref. #: WHA10347509 |
|
1.5 mL tubes |
Eppendorf |
Ref. #: 0030120086 |
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Software and algorithms |
|
|
|
ScanArray Express Microarray Analysis System |
PerkinElmer |
http://www.per
kinelmer.com/microarray |
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