A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds
Published: February 29, 2024
Abstract
Source: Moran, J. C. et al., Deferred Growth Inhibition Assay to Quantify the Effect of Bacteria-derived Antimicrobials on Competition. J. Vis. Exp. (2016)
This video showcases the deferred growth inhibition assay, used to investigate how antimicrobial compounds produced by one type of bacteria affect another competing bacterial strain.
Protocol
The following method is adapted to test competitor strains with inhibitor-producing strains. NOTE: This protocol involves aerosol generation with bacterial cultures. This can only be performed in a contained space with the implementation of locally approved safety considerations. Spraying of the culture onto the agar plates within a level 2 safety cabinet will minimize contamination of the agar plates and the surrounding environment. This will also protect the researcher …
Representative Results
Figure 1: The range of qualitative and quantitative results that can be obtained from the deferred growth inhibition assay. Results for the growth inhibition can range from (A) complete inhibition (score of 0), (B) partial inhibition (score of 1), (C) partial inhibition (score of 2) to (D) no inhibition…
Disclosures
The authors have nothing to disclose.
Materials
| Brain heart infusion broth | Lab M | lab049 | Rich general purpose media |
| Agar-agar | Merck Milipore | 101614 | |
| Petri dishes | Sarstedt | 82.1473.001 | |
| Plastic perfume vaporizer | not known | – | 10 cm high with 3.5 cm diameter clear plastic spray bottle with lid, purchased from the travel section of a supermarket |
| Universal bottles | no longer in production, alternative SLS BOT5006 | 28 ml glass cylindrical bottle (approx 280 mm diameter, 850 mm height), flat bottomed with plastic screw cap | |
| Decon 90 | Decon | surface active cleaning agent |
Tags
Cite This Article
A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds. J. Vis. Exp. (Pending Publication), e21993, doi: (2024).