A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples
Published: January 31, 2024
Abstract
Source: Balne, P. K., et al. Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles. J. Vis. Exp. (2017)
This video illustrates a bead-based multiplex assay for analyzing cytokines in human tears. The method allows for the simultaneous analysis of multiple cytokines from a single sample, providing enhanced sensitivity for studying immune responses in various pathophysiological conditions.
Protocol
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Tear Cytokine Analysis Collection of tears: Ask the subject to sit comfortably on an examination chair and place his/her head against the headrest. Ask the subject to look up, carefully open the Schirmer strips (made of Whatman filter paper no. 41) place the rounded end of the strip on the inferior fornix o…
Representative Results
Figure 1: Schematic flow diagram of tear cytokine profiling.
Disclosures
The authors have nothing to disclose.
Materials
| Milliplex MAP human cytokine / chemokine magnetic bead panel -1 kit | Merck, USA | HCYTOMAG-60K-41 | |
| Flexmap 3D luminex instrument | Luminex Corp, Austin, TX, USA | ||
| xPonent software | Luminex Corp, Austin, TX, USA | ||
| RBXGenerator software | BIO-RAD, France | ||
| Bio-Plex Manager 6.1 | BIO-RAD, France | ||
| Plate shaker | Corning, USA | ||
| TECAN Microplate Washer | Tecan, Switzerland | HydroSpeed | |
| Pipettes | Mettler Toledo, CA, USA | ||
| 1.5 ml microcentrifuge tube | Axygen, USA | MCT-150-C | |
| Schirmer tear flow test strip | Eye Care and Cure, USA | 101657 | |
| Flexmap 3D Calibration Kit | Luminex Corp, Austin, TX, USA | 40-028 | |
| Flexmap 3D Verification Kit | Luminex Corp, Austin, TX, USA | 40-029 | |
| Ocular examination chair |
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Cite This Article
A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples. J. Vis. Exp. (Pending Publication), e21906, doi: (2024).