A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples

Published: January 31, 2024

Abstract

Source: Balne, P. K., et al. Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles. J. Vis. Exp. (2017)

This video illustrates a bead-based multiplex assay for analyzing cytokines in human tears. The method allows for the simultaneous analysis of multiple cytokines from a single sample, providing enhanced sensitivity for studying immune responses in various pathophysiological conditions.

Protocol

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines. 1. Tear Cytokine Analysis Collection of tears: Ask the subject to sit comfortably on an examination chair and place his/her head against the headrest. Ask the subject to look up, carefully open the Schirmer strips (made of Whatman filter paper no. 41) place the rounded end of the strip on the inferior fornix o…

Representative Results

Figure 1: Schematic flow diagram of tear cytokine profiling.

Disclosures

The authors have nothing to disclose.

Materials

Milliplex MAP human cytokine / chemokine magnetic bead panel -1 kit Merck, USA HCYTOMAG-60K-41
Flexmap 3D luminex instrument  Luminex Corp, Austin, TX, USA
xPonent software Luminex Corp, Austin, TX, USA
RBXGenerator software BIO-RAD, France
Bio-Plex Manager 6.1 BIO-RAD, France
Plate shaker Corning, USA
TECAN Microplate Washer Tecan, Switzerland HydroSpeed
Pipettes Mettler Toledo, CA, USA
1.5 ml microcentrifuge tube Axygen, USA MCT-150-C
Schirmer tear flow test strip Eye Care and Cure, USA 101657
Flexmap 3D Calibration Kit Luminex Corp, Austin, TX, USA 40-028
Flexmap 3D Verification Kit Luminex Corp, Austin, TX, USA 40-029
Ocular examination chair

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Cite This Article
A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples. J. Vis. Exp. (Pending Publication), e21906, doi: (2024).

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