Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection
Published: July 31, 2023
Abstract
Source: Plaisance-Bonstaff, K., et al. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (2019)
In this video, we demonstrate the isolation of monocytes from whole human blood by immunomagnetic negative separation. The isolated monocytes can be used for further downstream analysis.
Protocol
All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Isolation of primary human monocytes by immunomagnetic negative selection Collect 40 mL of fresh, human whole blood (from either an HIV+ patient or healthy control) in four 10 mL ethylenediaminetetraacetic acid (EDTA) vacuum tubes (…
Disclosures
The authors have nothing to disclose.
Materials
| 0.5M EDTA | Invitrogen | AM9260G | |
| BD Vacutainer Plastic Blood Collection Tubes with K2EDTA | BD Biosciences | 366643 | |
| Easy 50 EasySep Magnet | StemCell Technologies | 18002 | |
| Easy Sep Direct Human Monocyte Isolation Kit | StemCell Technologies | 19669 | |
| PBS | Gibco | 20012027 | |
| RPMI 1640 with L-Glutamine | Corning | 10040CVMP |
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Cite This Article
Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection. J. Vis. Exp. (Pending Publication), e21512, doi: (2023).