Electroencephalography-Based Recording Technique to Record the Epileptiform Activity
Published: April 30, 2023
Abstract
Source: Carvalho, M. et al., A Model of Epileptogenesis in Rhinal Cortex-Hippocampus Organotypic Slice Cultures. J. Vis. Exp. (2021).
This video describes the technique for recording the epileptiform activity of rhinal cortex-hippocampus organotypic slices ex vivo using electrophysiology or EEG set-up. This helps to study the dynamics and progression of epileptogenesis and screen potential therapeutic targets to treat epilepsy.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of rhinal cortex-hippocampus slices NOTE: The preparation of rhinal cortex-hippocampus slices uses P6-7 Sprague-Dawley rats. Culture setup and medium preparation On the day before the culture, prepare the required media and place them at 4 °C. Prepare d…
Representative Results
Figure 1: Detailed procedure for the preparation of rhinal cortex-hippocampus organotypic slices. (A) Remove the brain from the head and place it in ice-cold GBSS with the dorsal surface faced up. (B) Insert the forceps into the cerebellum. Open the brain through the midline and remove the excess tissue over the hippocampus. (C) With a spatu…
Disclosures
The authors have nothing to disclose.
Materials
| Artificial cerebrospinal fluid (aCSF) | Homemade | ||
| B-27™ Supplement (50X), serum free | Thermo Fisher Scientific | 17504-044 | |
| Blades for scalpel handle | Fine Science Tools | 10011-00 | |
| Bovine Serum Albumin (BSA) | NZYTech | MB04602 | 5% BSA is used to dilute the primary antibodies. Add 0.5g BSA in 10 mL PBS. |
| Brain/Tissue Slice Chamber System | Warner Instruments | ||
| Cell culture inserts, 30 mm, hydrophilic PTFE | Millipore SAS | PICM03050 | |
| Conventional incubator | Thermo Scientific Heraeus | BB15, Function Line | Set to 37 °C and 5% CO2 |
| Gey’s Balanced Salt Solution (GBSS) | Biological Industries | 01-919-1A | |
| Glass Electrodes | Science Products | GB150F-10 | Round tips homemade |
| Interface chamber | Warner Instruments | BSC-HT Haas Top | |
| Iris Spatula Curved | Fine Science Tools | 10092-12 | |
| Lens Cleaning Paper | TIFFEN | ||
| L-Glutamine solution 200 mM (Q) | Thermo Fisher Scientific | 25030-024 | |
| Tissue Chopper | The Mickle Laboratory Engineering CO. LTD. | MTC/2 | Set to 350 μm |
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Cite This Article
Electroencephalography-Based Recording Technique to Record the Epileptiform Activity. J. Vis. Exp. (Pending Publication), e21282, doi: (2023).