This video describes a procedure for target gene knockout using a tamoxifen-inducible Cre-recombinase system following intraperitoneal injection of tamoxifen in a mouse model.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Induction of Transfected CreER with Tamoxifen CAUTION: Tamoxifen is harmful, may be cancerous or damage fertility. Please refer to the safety data sheet. Plan intraperitoneal tamoxifen injections on three consecutive days. On day 1, dissolve 10 mg of tamoxifen in 40…
CreER-LoxP System-Based Target Gene Inactivation: A Tamoxifen-Inducible Cre Recombinase System for Target Gene Knockout in a Mouse Model. J. Vis. Exp. (Pending Publication), e20976, doi: (2023).