Modeling Preservation Injury in Porcine Donor Kidney: A Procedure to Induce Graft Dysfunction by Subjecting Kidney to Prolonged Cold Ischemia

Published: April 30, 2023

Abstract

Source: Liu, W. J., et al. Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation And Continuous Telemetry. J. Vis. Exp. (2020)

In this video, we describe a technique to induce preservation injury via the prolonged cold ischemic storage time in porcine donor kidney.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Back-table and organ preservation After graft retrieval, immediately cannulate the renal artery using a standard 14 G (orange) peripheral catheter and fix it using a tourniquet prepared from 3-0 polyglactin. Rinse the kidney with a cold organ preservation solution. After flushing with 500 mL of organ preservation sol…

Disclosures

The authors have nothing to disclose.

Materials

Organ preservation solution (e.g. HTK) Dr. Franz Köhler Chemie GmbH, Bensheim, Germany Should be decided based on preference and experimental design Organ preservation
Vessel loops Medline International Germany GmbH,Kleve, Germany VLMINB Hold and adjust the vessel
Sterile organ bag Raguse Gesellschaft für medizinische Produkte, Ascheberg, Germany 800059 Organ preservation
Micro steel needle holder straight 0,5mm, with spring lock ASANUS Medizintechnik GmbH, Tuttlingen, Germany MN1324D Microsurgical needle holder
3M™ Standard Surgical Mask 1810F 3M Deutschland GmbH, Neuss, Germany 3M-ID 7000039767 Surgical mask
VICRYL – ligatures Sutupak purple braided, 3-0 Johnson & Johnson Medical GmbH – Ethicon Deutschland, Norderstedt, Germany V1215E Threads for ligature
Prolene 3-0,blue monofil,FS1 needle Johnson & Johnson Medical GmbH – Ethicon Deutschland, Norderstedt, Germany EH7694H Skin

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Cite This Article
Modeling Preservation Injury in Porcine Donor Kidney: A Procedure to Induce Graft Dysfunction by Subjecting Kidney to Prolonged Cold Ischemia. J. Vis. Exp. (Pending Publication), e20913, doi: (2023).

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