Pancreatic Intravital Imaging Window Installation in Mouse Model: An Integrated Pancreatic Imaging Window for Time-lapse In Vivo Imaging of Murine Pancreas Microstructure
Published: April 30, 2023
Abstract
Source: Park, I. et. al. Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window. J. Vis. Exp. (2021)
This video demonstrates the procedure to implant a pancreatic imaging window to separate the pancreas from the intestines for longitudinal time-lapse high-resolution imaging of the pancreas microstructure.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Surgery Prepare a sterile surgical platform and sterilize the surfaces with 70% ethanol.| NOTE: For longitudinal imaging sessions, aseptic technique is essential. Anesthetize mice with a mixture of tiletamine/zolazepam (30 mg/kg) and xylazine (10 mg/kg). NOTE: Use of tiletamine/z…
Representative Results
Figure 1. Design and photograph of the pancreatic intravital imaging window. (A) A 3D and cross-sectional view of the pancreatic intravital imaging window. (B) Anterior and posterior photograph of the pancreatic imaging window.
Disclosures
The authors have nothing to disclose.
Materials
| Cover glasses circular | Marienfeld | 111520 | Cover glass for pancreatic imaging window |
| IRIS Scissor | JEUNGDO BIO & PLANT CO, LTD | S-1107-10 | This product can be replaced with the product from other company |
| Loctite 401 | Henkel | 401 | N-butyl cyanoacrylate glue |
| Micro Needle holder | JEUNGDO BIO & PLANT CO, LTD | H-1126-10 | This product can be replaced with the product from other company |
| Micro rectractor | JEUNGDO BIO & PLANT CO, LTD | 17004-03 | This product can be replaced with the product from other company |
| Microforceps | JEUNGDO BIO & PLANT CO, LTD | F-1034 | This product can be replaced with the product from other company |
| Nylon 4-0 | AILEE | NB434 | Non-Absorbable Suture |
| Omnican N 100 30G | B BRAUN | FT9172220S | For Vascular Catheter, Use only Needle part |
| Pancreatic imaging window | Geumto Engineering | Custom order | Pancreatic imaging window – custom order |
| Physiosuite | Kent Scientific | PS-02 | Homeothermic temperature controller |
| Ring Forceps | JEUNGDO BIO & PLANT CO, LTD | F-1090-3 | This product can be replaced with the product from other company |
| Rompun | Bayer | Rompun | Anesthetic agent |
| Zoletil | Virbac | Zoletil 100 | Anesthetic agent |
| BALB/C Nude | OrientBio | BALB/C Nude | BALB/C Nude |
Tags
Cite This Article
Pancreatic Intravital Imaging Window Installation in Mouse Model: An Integrated Pancreatic Imaging Window for Time-lapse In Vivo Imaging of Murine Pancreas Microstructure. J. Vis. Exp. (Pending Publication), e20796, doi: (2023).