Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo
Published: April 30, 2023
Abstract
Source: McKee, Robert A. et al. Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury. J. Vis. Exp. (2016)
This video demonstrates the intravenous microinjection of nephrotoxins into the tail vein of a zebrafish embryo to study their chemical effects on renal function.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Solutions Make a 50x stock solution of E3 embryo media by mixing 73.0 g NaCl, 3.15 g KCl, 9.15 g CaCl2, and 9.95 g MgSO4 in 5 L of distilled water, and store at RT. Make a 50x stock solution of E3 embryo media by mixing 73.0 g NaCl, 3.15 g KCl, 9.15 g CaCl2,…
Disclosures
The authors have nothing to disclose.
Materials
| Sodium Chloride | American Bioanalytical | AB01915 | |
| Potassium Chloride | American Bioanalytical | AB01652 | |
| Magnesium Sulfate | Sigma-Aldrich | M7506 | |
| Calcium Chloride | American Bioanalytical | AB00366 | |
| N-Phenylthiourea (PTU) | Aldrich Chemistry | P7629 | |
| Ethyl 3-aminobenzoate (Tricaine) | Fluka Analytical | A5040 | |
| Borosilicate glass | Sutter Instruments Co. | BF100-50-10 | |
| Flaming/Brown Micropipette puller | Sutter Instruments Co. | Mo. P097 | |
| UltraPure Agarose | Invitrogen | 15510-027 | |
| Falcon Diposable Petri Dishes, Sterile, Corning: | |||
| 60 mm x 15 mm | VWR | 25373-085 | |
| 100 mm x 15 mm | VWR | 25373-100 | |
| Microinjection tray 150 mm x 15 mm | VWR | 25373-187 | |
| Low Temperature Incubator | Fischer Scientific | 11 690 516DQ | |
| Micro Dissecting Tweezer | Roboz Surgical Instruments Co. | RS-5010 | |
| Micrometer | Ted Pella, Inc. | 2280-24 |
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Cite This Article
Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo. J. Vis. Exp. (Pending Publication), e20572, doi: (2023).