Mouse Headpost Implantation: A Method for Head Fixation in Rodents
Published: April 30, 2023
Abstract
Source: França de Barros, F., et al. Long-term Sensory Conflict in Freely Behaving Mice. J. Vis. Exp. (2019).
This video shows a headpost implantation surgery in murine model. The headpost is used not only to head-fix and therefore restrain the mouse during experimentation, but also to securely attach a head device in freely moving animals.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Headpost Implantation Surgery NOTE: Ensure the use of sterile instruments and arrange surgery and recovery in different zones. Once mastered, the implantation procedure lasts about 30 min. For analgesia, 30 min before the beginning of the surgery, subcutaneously inject bupren…
Materials
| Blunt scissors | FST | 14079-10 | |
| Catalyst V | Sun Medical, Japan | LX22 | Parkell bio-materials, Kit n°S380 |
| Dentalon Plus | Heraeus | 37041 | |
| Green activator | Sun Medical, Japan | VE-1 | Parkell bio-materials, Kit n°S380 |
| Monomer | Sun Medical, Japan | MF-1 | Parkell bio-materials, Kit n°S380 |
| Ocrygel | TvmLab | 10779 | Ophtalmic vet ointment |
| Polymer L-type clear (cement) | Sun Medical, Japan | TT12F | Parkell bio-materials, Kit n°S380 |
Tags
Cite This Article
Mouse Headpost Implantation: A Method for Head Fixation in Rodents. J. Vis. Exp. (Pending Publication), e20090, doi: (2023).