JOVE JOURNAL

遗传学

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Journal (Genetics)

Preparation of 96-Well Plate for Monochrome Multiplex Quantitative PCR (MMqPCR) for Telomere Length Measurement

Noelle A. Martin*¹, Lauren W. Y. McLester-Davis*^2,3,4, Trevor R. Roy¹, Madeline G. Magruder⁵, Waylon J. Hastings¹, Stacy S. Drury⁶

¹School of Medicine,Tulane University, ²Native American Center for Health Professions,University of Wisconsin-Madison, ³Department of Medicine,University of Wisconsin-Madison, ⁴Department of Biochemistry,University of Wisconsin-Madison, ⁵School of Science and Engineering,Tulane University, ⁶Department of Psychiatry and Behavioral Sciences,Boston Children’s Hospital

Journal (Genetics)

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation

Daniel J. Davis^1,2,4, James F. McNew², Jennifer N. Walls³, Christine E. Bethune³, Payton S. Oswalt³, Elizabeth C. Bryda^1,2,3,4

¹Animal Modeling Core,University of Missouri, ²Comparative Medicine Program,University of Missouri, ³Rat Resource and Research Center,University of Missouri, ⁴Department of Veterinary Pathobiology,University of Missouri

Journal (Genetics)

AQRNA-seq for Quantifying Small RNAs

Ruixi Chen¹, Daniel Yim², Peter C. Dedon^1,2

¹Department of Biological Engineering,Massachusetts Institute of Technology, ²Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

Journal (Genetics)

Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation

Ruixi Chen¹, Daniel Yim², Peter C. Dedon^1,2

¹Department of Biological Engineering,Massachusetts Institute of Technology, ²Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

Journal (Genetics)

Cryopreservation of Primordial Germ Cells Collected from Drosophila Donor Embryos

Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

Journal (Genetics)

Thawing and Transplantation of Cryopreserved Primordial Germ Cells for Drosophila Strain Revival

Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

Journal (Genetics)

Primordial Germ Cell Cryopreservation and Revival of Drosophila Strains

Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

Journal (Genetics)

Collection of Primordial Germ Cells from Drosophila Donor Embryos

Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka³, Satoru Kobayashi², Toshiyuki Takano-Shimizu-Kouno¹

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

Journal (Genetics)

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Mariia Metelova¹, Rikke Rejnholdt Jensen¹, Nils Krietenstein¹

¹Novo Nordisk Foundation Center for Protein Research,University of Copenhagen

Journal (Genetics)

Daily Transfers, Archiving Populations, and Measuring Fitness in the Long-Term Evolution Experiment with Escherichia coli

Jeffrey E. Barrick^1,2, Zachary D. Blount^2,3, Devin M. Lake^2,4, Jack H. Dwenger¹, Jesus E. Chavarria-Palma¹, Minako Izutsu^2,3, Michael J. Wiser^2,5

¹Department of Molecular Biosciences,The University of Texas at Austin, ²BEACON Center for the Study of Evolution in Action,Michigan State University, ³Department of Microbiology and Molecular Genetics,Michigan State University, ⁴Department of Integrative Biology,Michigan State University, ⁵Biological Sciences Program,Michigan State University

Journal (Genetics)

Preparation for Electroporation and Ribonucleoprotein Delivery for Genome Editing in Mouse Bone Marrow-Derived Macrophages

Julia Craft¹, Tina Truong¹, Bennett H. Penn^1,2

¹Department of Internal Medicine,University of California, Davis, ²Department of Medical Microbiology and Immunology,University of California, Davis

Journal (Genetics)

High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes

Julia Craft¹, Tina Truong¹, Bennett H. Penn^1,2

¹Department of Internal Medicine,University of California, Davis, ²Department of Medical Microbiology and Immunology,University of California, Davis

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