JOVE JOURNAL

生物学

Showing 901 - 912 in 3,291 videos.

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

Yen Xuan Ngo^1,2,3, Kenta Haga⁴, Ayako Suzuki⁴, Hiroko Kato⁴, Hiromi Yanagisawa^1,5, Kenji Izumi⁴, Aiko Sada^1,3

¹Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ²Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba, ³International Research Center for Medical Sciences (IRCMS),Kumamoto University, ⁴Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences,Niigata University, ⁵Faculty of Medicine,University of Tsukuba

Journal (Biology)

Hyperactive piggyBac Transposase-mediated Germline Transformation in the Fall Armyworm, Spodoptera frugiperda

Xien Chen¹, Subba Reddy Palli¹

¹Department of Entomology, College of Agriculture, Food and Environment,University of Kentucky

Journal (Biology)

Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing

Janna Leiz*^1,2, Christian Hinze*^1,2, Anastasiya Boltengagen³, Caroline Braeuning⁴, Christine Kocks³, Nikolaus Rajewsky³, Kai M. Schmidt-Ott^1,2

¹Department of Nephrology and Medical Intensive Care Medicine,Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, ²Molecular and Translational Kidney Research,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ³Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), ⁴Berlin Institute of Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)

Journal (Biology)

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Madalena R. de Almeida¹, Eduardo Gameiro¹, Sérgio F. de Almeida¹, Robert M. Martin¹

¹Instituto de Medicina Molecular João Lobo Antunes,Faculdade de Medicina da Universidade de Lisboa

Journal (Biology)

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Aline Stedman^1,3, Antonin Levy^1,4, Philippe J. Sansonetti^1,2,5, Giulia Nigro^1,6

¹Molecular Microbial Pathogenesis Unit,Institut Pasteur, ²Chaire de Microbiologie et Maladies Infectieuses,Collège de France, ³Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit,Sorbonne Université, CNRS UMR7622, INSERM U1156, ⁴Molecular Radiotherapy, INSERM U1030, Gustave Roussy,Université Paris-Saclay, ⁵The Center for Microbes, Development and Health,Institut Pasteur Shanghai and Chinese Academy of Sciences, ⁶Microenvironment and Immunity Unit,Institut Pasteur

Journal (Biology)

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Alan Y. Hsu¹, Zhicheng Peng¹, Hongbo Luo¹, Fabien Loison^1,2

¹Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, ²Department of Microbiology, Faculty of Science,Mahidol University

Journal (Biology)

Detecting Virus and Salivary Proteins of a Leafhopper Vector in the Plant Host

Yanfei Wang¹, Xin Wang¹, Zhiqiang Li¹, Qian Chen¹

¹Vector-borne Virus Research Center, Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology,Fujian Agriculture and Forestry University

Journal (Biology)

Automated Detection and Analysis of Exocytosis

Fabio Urbina¹, Stephanie L. Gupton¹

¹UNC Neuroscience Center, Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill

Journal (Biology)

Planarian Ovary Dissection for Ultrastructural Analysis and Antibody Staining

Fengli Guo¹, Melainia McClain¹, Xia Zhao¹, Kexi Yi¹, Tari Parmely¹, Jay Unruh¹, Brian Slaughter¹, Leonid Kruglyak^2,3, Longhua Guo^2,3, Alejandro Sánchez Alvarado^1,2

¹Stowers Institute for Medical Research, ²Howard Hughes Medical Institute, ³Department of Human Genetics,University of California Los Angeles

Journal (Biology)

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds

Josselin Rigaill*^1,2, Estelle Audoux*¹, Killian Rodriguez¹, Aurélien Peyron¹, Philippe Berthelot^1,3, Jérôme Josse^4,5, Frédéric Laurent^4,5, Robin Caire¹, Paul O. Verhoeven^1,2

¹CIRI, Centre International de Recherche en Infectiologie, GIMAP team,University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, ²Department of Infectious Agents and Hygiene,University Hospital of St-Etienne, St-Etienne, France, ³Department of Infectious Diseases,University Hospital of St-Etienne, St-Etienne, France, ⁴CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team,University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, ⁵Department of Bacteriology, Institute for Infectious Agents,Hospices Civils de Lyon, Lyon, France

Journal (Biology)

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

Cyril Scandola¹, François Lanza¹, Christian Gachet¹, Anita Eckly¹

¹Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

Journal (Biology)

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Maria I. Lazaro-Pena¹, Adam B. Cornwell¹, Andrew V. Samuelson¹

¹Department of Biomedical Genetics,University of Rochester Medical Center

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