JOVE JOURNAL

生物化学

Showing 577 - 588 in 778 videos.

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Katharina Kohl¹, Thomas Fleming^2,3, Kübra Acunman¹, Hans-Peter Hammes^1,4, Michael Morcos*¹, Andrea Schlotterer*¹

¹5th Medical Department, Medical Faculty Mannheim,Heidelberg University, ²Department of Internal Medicine,Heidelberg University, ³German Center for Diabetes Research (DZD), ⁴European Center for Angioscience, Medical Faculty Mannheim,Heidelberg University

Journal (Biochemistry)

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Xuechun Feng¹, Nannan Liu¹

¹Department of Entomology and Plant Pathology,Auburn University

Journal (Biochemistry)

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Keri L Colabroy¹, Katlyn Mayer¹

¹Department of Chemistry,Muhlenberg College

Journal (Biochemistry)

Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis

Markus Nachbar¹, Jorrit Maul¹, Matthias Stein¹, Hermann Wätzig¹

¹Institute of Medicinal and Pharmaceutical Chemistry,TU Braunschweig

Journal (Biochemistry)

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

Xiongwei Cai^1,2,3, Yi Zheng¹, Nancy A. Speck²

¹Division of Experimental Hematology,Cincinnati Children's Hospital Medical Center, ²Department of Cell and Developmental Biology, Abramson Family Cancer Research Institute,Perelman School of Medicine, University of Pennsylvania, ³Department of Obstetrics and Gynecology, Southwest Hospital,Third Military Medical University

Journal (Biochemistry)

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

Xiaoying Qi*¹, Yunyun Zhang*², Yuan Zhang¹, Tianhui Ni³, Wenfeng Zhang², Chunhua Yang¹, Jia Mi^1,4, Jiandi Zhang^2,3, Geng Tian¹

¹Medicine and Pharmacy Research Center,Binzhou Medical University, ²Yantai Zestern Biotechnique Co. LTD, ³Precision Medicine research center,Binzhou Medical University, ⁴Department of Chemistry - BMC,Uppsala University

Journal (Biochemistry)

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Sayan Chakraborty¹, Krittin Trihemasava¹, Guozhou Xu¹

¹Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences,North Carolina State University

Journal (Biochemistry)

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Tanja D. Becke^1,2,3, Stefan Ness², Stefanie Sudhop^1,3, Hermann E. Gaub³, Markus Hilleringmann², Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*^1,3

¹Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, ³Center for Nano Science,Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Journal (Biochemistry)

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Valentina Iadevaia*¹, Ana M. Matia-González*¹, André P. Gerber¹

¹Dept. of Microbial Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences,University of Surrey

Journal (Biochemistry)

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale

Michael J. Stephenson¹, James Reed¹, Bastiaan Brouwer¹, Anne Osbourn¹

¹John Innes Centre

Journal (Biochemistry)

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Alicia Ventresca Miller¹, Ricardo Fernandes^1,2, Anneke Janzen¹, Ayushi Nayak¹, Jillian Swift¹, Jana Zech¹, Nicole Boivin¹, Patrick Roberts¹

¹Max Planck Institute for the Science of Human History, ²School of Archaeology,University of Oxford

Journal (Biochemistry)

Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering

Daniela Baitan^1,2, Robin Schubert^2,3, Arne Meyer¹, Karsten Dierks¹, Markus Perbandt^2,3, Christian Betzel^2,3

¹Xtal Concepts GmbH, ²Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation c/o DESY,University of Hamburg, ³The Hamburg Center for Ultrafast Imaging,University of Hamburg

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