JOVE JOURNAL

生物化学

Showing 373 - 384 in 778 videos.

PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase

Eduardo Hilario¹, Li Fan², Leonard J. Mueller¹, Michael F. Dunn²

¹Department of Chemistry,University of California-Riverside, ²Department of Biochemistry,University of California-Riverside

Journal (Biochemistry)

Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology

Sayuri L. Higashi^1,2, Kazuya Yagyu^1,2, Haruna Nagase^1,2, Craig S. Pearson¹, Herbert M. Geller¹, Yasuhiro Katagiri¹

¹Laboratory of Developmental Neurobiology, Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute,National Institutes of Health, ²United Graduate School of Drug Discovery and Medical Information Sciences,Gifu University

Journal (Biochemistry)

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

Wei Zhou¹, Hiroyuki Takeda²

¹Graduate School of Medicine,Ehime University, ²Proteo-Science Center,Ehime University

Journal (Biochemistry)

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Ragini M. Mistry^1,2, Pankaj K. Singh^1,3, Maureen G. Mancini^1,2, Fabio Stossi^1,2, Michael A. Mancini^1,2,3,4

¹GCC Center for Advanced Microscopy and Image Informatics, ²Department of Molecular and Cellular Biology,Baylor College of Medicine, ³Center for Translational Cancer Research, Institute of Biosciences and Technology,Texas A&M University, ⁴Department of Pharmacology and Chemical Biology,Baylor College of Medicine

Journal (Biochemistry)

Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue

Sanaz Dastghaib^1,2, Zahra Shahsavar³, Zahra Karimian⁴, Pooneh Mokarram^1,5

¹Department of Biochemistry, School of Medicine,Shiraz University of Medical Sciences, ²Endocrinology and Metabolism Research Center, Nemazee Hospital,Shiraz University of Medical Sciences, ³English Language Department, Faculty of Paramedical Sciences,Shiraz University of Medical Sciences, ⁴Department of e-Learning in Medical Sciences, Virtual School and Center of Excellence in e-Learning,Shiraz University of Medical Sciences, ⁵Autophagy Research Center, School of Medicine,Shiraz University of Medical Sciences

Journal (Biochemistry)

An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics

Anthony Gaba*¹, Hongyun Wang*¹, Xiaohui Qu¹

¹Molecular Biology Program,Memorial Sloan Kettering Cancer Center

Journal (Biochemistry)

A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics

Yifan Ge¹, Sivakumar Boopathy¹, Adam Smith², Luke H. Chao^1,3

¹Department of Molecular Biology,Massachusetts General Hospital, ²Department of Chemistry,University of Akron, ³Department of Genetics,Harvard Medical School

Journal (Biochemistry)

Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

Sandeep K. Misra¹, Joshua S. Sharp^1,2,3

¹Department of Biomolecular Sciences,University of Mississippi, ²Department of Chemistry and Biochemistry,University of Mississippi, ³GenNext Technologies, Inc.

Journal (Biochemistry)

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Kyle G. Kroeck^1,2, Weihua Qiu^1,2, Claudio Catalano^1,2, Thi Kim Hoang Trinh^1,2, Youzhong Guo^1,2

¹Department of Medicinal Chemistry, School of Pharmacy,Virginia Commonwealth University, ²Institute for Structural Biology, Drug Discovery and Development, School of Pharmacy,Virginia Commonwealth University

Journal (Biochemistry)

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Varsha Tirumalai^1,2, Melvin Prasad¹, P. V. Shivaprasad¹

¹National Centre for Biological Sciences,Tata Institute of Fundamental Research, ²SASTRA University

Journal (Biochemistry)

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Inés Medina-Lozano*^1,4, Juan Ramón Bertolín*^2,4, Raquel Zufiaurre³, Aurora Díaz^1,4

¹Unidad de Hortofruticultura,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ²Unidad de Producción y Sanidad Animal,Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ³Dpto Química Analítica,Escuela Politécnica Superior (Universidad de Zaragoza), ⁴Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza)

Journal (Biochemistry)

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Philipp Gaugler¹, Verena Gaugler¹, Marília Kamleitner¹, Gabriel Schaaf¹

¹Department of Plant Nutrition, Institute of Crop Science and Resource Conservation,University of Bonn

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