JOVE JOURNAL

生物化学

Showing 361 - 372 in 731 videos.

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Alexandria Slayden, Kelsey North, Shivantika Bisen, Alex M. Dopico, Anna N. Bukiya, Avia Rosenhouse-Dantsker

¹Department of Pharmacology, Addiction Science and Toxicology,The University of Tennessee HSC, ²Department of Chemistry,University of Illinois at Chicago

Journal (Biochemistry)

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Karel Bezstarosti, Lennart van der Wal, Jeroen A. A. Demmers

¹Proteomics Center,Erasmus University Medical Center

Journal (Biochemistry)

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Ying Fan*¹, Francesca Tonelli*¹, Shalini Padmanabhan, Marco A.S. Baptista, Lindsey Riley, Danielle Smith, Connie Marras, Andrew Howden, Dario R. Alessi, Esther Sammler⁶

¹MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, ²The Michael J. Fox Foundation for Parkinson's Research, ³Department of Medical and Molecular Genetics,Indiana University School of Medicine, ⁴Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital,University of Toronto, ⁵Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, ⁶Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee

Journal (Biochemistry)

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Filip Pamula², Jonas Mühle, Alain Blanc, Rony Nehmé, Patricia C. Edwards, Christopher G. Tate, Ching-Ju Tsai

¹Laboratory of Biomolecular Research,Paul Scherrer Institute, ²Department of Biology,ETH Zürich, ³Center for Radiopharmaceutical Sciences,Paul Scherrer Institute, ⁴Laboratory of Molecular Biology,Medical Research Council

Journal (Biochemistry)

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Hayley Abbiss^2,3, Joel P. A. Gummer⁴, Michael Francki⁶, Robert D. Trengove

¹Research and Innovation,Murdoch University, ²Centre for Digital Agriculture,Curtin University, ³Centre for Integrative Metabolomics and Computational Biology, School of Science,Edith Cowan University, ⁴Centre for Computational and System Medicine, Health Futures Institute,Murdoch University, ⁵Department of Primary Industries and Regional Development, ⁶State Agricultural Biotechnology Centre,Murdoch University

Journal (Biochemistry)

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

Damian Jacenik, Marta Zielińska, Sylwia Michlewska, Jakub Fichna, Wanda M. Krajewska

¹Department of Cytobiochemistry, Faculty of Biology and Environmental Protection,University of Lodz, ²Department of Biochemistry, Faculty of Medicine,Medical University of Lodz, ³Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection,University of Lodz

Journal (Biochemistry)

Characterizing Cellular Proteins with In-cell Fast Photochemical Oxidation of Proteins

Emily E. Chea, Aimee Rinas, Jessica A. Espino, Lisa M. Jones

¹Department of Pharmaceutical Sciences,University of Maryland Baltimore, ²AIT Bioscience

Journal (Biochemistry)

Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay

Manel Zeghal, Geneviève Laroche, Patrick M. Giguère²

¹Department of Biochemistry, Microbiology and Immunology,University of Ottawa, ²Brain and Mind Research Institute,University of Ottawa

Journal (Biochemistry)

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Justin R. Welden, Anna Pawluchin, Jacob van Doorn, Stefan Stamm

¹Institute for Molecular and Cellular Biochemistry,University of Kentucky

Journal (Biochemistry)

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Karamat Mohammad, Heng Jiang, Md. Israil Hossain, Vladimir I. Titorenko

¹Department of Biology,Concordia University, ²Department of Chemistry and Biochemistry, Centre for Biological Applications of Mass Spectrometry,Concordia University

Journal (Biochemistry)

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Judith A. Stolwijk, Anne-Kathrin Mildner, Christian Kade, Michael Skiba, Guenther Bernhardt, Armin Buschauer, Harald Huebner, Peter Gmeiner, Joachim Wegener⁴

¹Institute of Analytical Chemistry, Chemo- and Biosensors,University of Regensburg, ²Institute of Pharmacy,University of Regensburg, ³Department of Chemistry and Pharmacy,Friedrich-Alexander University Erlangen-Nuernberg, ⁴Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

Journal (Biochemistry)

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Sen Takeda, Kentaro Yoshimura, Hiroshi Tanihata

¹Department of Anatomy and Cell Biology,University of Yamanashi School of Medicine, ²Shimadzu Corporation

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