JOVE JOURNAL

生物化学

Showing 133 - 144 in 776 videos.

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Journal (Biochemistry)

Generating Submitochondrial Vesicles from Saccharomyces cerevisiae Mitochondria to Isolate Mitochondrial Contact Sites

Siavash Khosravi*^1,2, Johanna Frickel*¹, Max E. Harner¹

¹Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, ²Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Journal (Biochemistry)

Separation and Analysis of Submitochondrial Vesicles to Identify Mitochondrial Contact Site Proteins

Siavash Khosravi*^1,2, Johanna Frickel*¹, Max E. Harner¹

¹Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, ²Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Journal (Biochemistry)

An Improved Method to Isolate Mitochondrial Contact Sites

Siavash Khosravi*^1,2, Johanna Frickel*¹, Max E. Harner¹

¹Department of Cell Biology, Biomedical Center, Medical Faculty,Ludwig-Maximilians University Munich, ²Cardio-Metabolic Diseases,Boehringer Ingelheim Pharma GmbH & Co. KG

Journal (Biochemistry)

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Pavan S. Krishnan^1,2, Fernando T. Zamuner^1,3, Carolyn M. Jenks¹, Johnny Y. Xie⁴, Lisa Zhang⁵, Mohammed Lehar¹, Neal S. Fedarko⁶, Mariana Brait^1,3, John P. Carey¹

¹Department of Otolaryngology-Head & Neck Surgery,Johns Hopkins University School of Medicine, ²Virginia Commonwealth University School of Medicine, ³Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center,Johns Hopkins University School of Medicine, ⁴Department of Otolaryngology-Head and Neck Surgery,University of Michigan Medical School, ⁵Department of Otolaryngology-Head and Neck Surgery,The Ohio State University College of Medicine, ⁶ICTR Clinical Research Core Laboratory,Johns Hopkins University School of Medicine

Journal (Biochemistry)

Running the mAbscale Application to Calculate the Masses of Monoclonal Antibody-Based Protein Therapeutics

Thomas Harkins^1,2, Li Cao¹, Kshitij Khatri^1,3

¹GlaxoSmithKline, ²Merck, ³Moderna

Journal (Biochemistry)

An Open-Source Framework for Mass Calculation of Antibody-Based Therapeutic Molecules

Thomas Harkins^1,2, Li Cao¹, Kshitij Khatri^1,3

¹GlaxoSmithKline, ²Merck, ³Moderna

Journal (Biochemistry)

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Journal (Biochemistry)

Image Acquisition of Lipid Droplets and Analysis

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Journal (Biochemistry)

Analysis of Fluorescent-Stained Lipid Droplets with 3D Reconstruction for Hepatic Steatosis Assessment

Karina Garcia^1,2,3,4, André Alves^1,2,3, Teresa M. Ribeiro-Rodrigues^1,2,3, Flávio Reis^1,2,3, Sofia Viana^1,2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

Journal (Biochemistry)

Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus

Mingqing Tang^1,2, Hongjun Lin¹, Chengjia Hu¹, Hui Yan^1,2

¹Medicine School,Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Journal (Biochemistry)

Protein Isolation from Sipunculus nudus

Mingqing Tang^1,2, Hongjun Lin¹, Chengjia Hu¹, Hui Yan^1,2

¹Medicine School,Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

Journal (Biochemistry)

Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts

Mingqing Tang^1,2, Hongjun Lin¹, Chengjia Hu¹, Hui Yan^1,2

¹Medicine School,Huaqiao University, ²Engineering Research Centre of Molecular Medicine of Ministry of Education

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