JOVE JOURNAL

生物化学

Showing 697 - 708 in 776 videos.

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Journal (Biochemistry)

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Meong Il Kim¹, Choongdeok Lee¹, Minsun Hong¹

¹Division of Biological Science and Technology,Yonsei University

Journal (Biochemistry)

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

Toshio Moriya¹, Michael Saur¹, Markus Stabrin¹, Felipe Merino¹, Horatiu Voicu², Zhong Huang², Pawel A. Penczek², Stefan Raunser¹, Christos Gatsogiannis¹

¹Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, ²Department of Biochemistry and Molecular Biology,The University of Texas Medical School at Houston

Journal (Biochemistry)

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Junyan Ma¹, Inna S. Yanez-Orozco², Soheila Rezaei Adariani², Drew Dolino³, Vasanthi Jayaraman³, Hugo Sanabria²

¹Department of Chemistry,Clemson University, ²Department of Physics and Astronomy,Clemson University, ³Department of Biochemistry and Molecular Biology, Center for Membrane Biology, Graduate School for Biomedical Sciences,University of Texas Health Science Center

Journal (Biochemistry)

Expression, Purification, and Antimicrobial Activity of S100A12

Emmanuel Jackson¹, Saffron Little¹, Dana S. Franklin¹, Jennifer A. Gaddy^2,3, Steven M. Damo^1,4

¹Department of Life and Physical Sciences,Fisk University, ²Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs, ³Department of Medicine - Division of Infectious Diseases,Vanderbilt University Medical School, ⁴Department of Biochemistry,Vanderbilt University

Journal (Biochemistry)

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization

Xavier Morató*^1,2, Marc López-Cano*^1,2, Paula M. Canas³, Rodrigo A. Cunha³, Francisco Ciruela^1,2

¹Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL,Universitat de Barcelona, L'Hospitalet de Llobregat, ²Institut de Neurociències,Universitat de Barcelona, ³Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine,University of Coimbra

Journal (Biochemistry)

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Tyler Rhinesmith¹, Bryan A. Killinger², Akhil Sharma³, Anna Moszczynska³

¹Physiology,Michigan State University, ²Center for Neurodegenerative Science Van Andel Institute, ³Pharmaceutical Sciences,Wayne State University

Journal (Biochemistry)

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Melissa Bredow¹, Heather E. Tomalty¹, Virginia K. Walker^1,2,3

¹Department of Biology,Queen's University, ²Department of Biomedical and Molecular Sciences,Queen's University, ³School of Environmental Sciences,Queen's University

Journal (Biochemistry)

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Christina D. King¹, Joseph D. Dudenhoeffer¹, Liqing Gu², Adam R. Evans³, Renã A. S. Robinson¹

¹Department of Chemistry,University of Pittsburgh, ²SGS North America Inc., ³Large Molecule Analytical Development, Pharmaceutical Development & Manufacturing Science,Janssen Research and Development

Journal (Biochemistry)

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors

Tobias G. Kapp*¹, Maximilian Fottner*¹, Horst Kessler¹

¹Department of Chemistry,Technische Universität München

Journal (Biochemistry)

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Javier Barallobre-Barreiro¹, Ferheen Baig¹, Marika Fava¹, Xiaoke Yin¹, Manuel Mayr¹

¹King's British Heart Foundation Centre,King's College London

Journal (Biochemistry)

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Danlin Yang¹, Ajit Singh², Helen Wu¹, Rachel Kroe-Barrett¹

¹Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., ²The Fu Foundation School of Engineering and Applied Science,Columbia University

Journal (Biochemistry)

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Kathrin Rösch¹, Marcel Kwiatkowski², Hartmut Schlüter², Eva Herker¹

¹Heinrich Pette Institute,Leibniz Institute for Experimental Virology, ²Core Facility Mass Spectrometric Proteomics,University Medical Center Hamburg-Eppendorf

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