JOVE JOURNAL

生物化学

Showing 289 - 300 in 776 videos.

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Journal (Biochemistry)

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Olivia Roscow¹, Wei Zhang¹

¹Department of Molecular and Cellular Biology, College of Biological Science,University of Guelph

Journal (Biochemistry)

Spectrophotometric Methods for the Study of Eukaryotic Glycogen Metabolism

Wayne A. Wilson¹

¹Department of Biochemistry & Nutrition,Des Moines University

Journal (Biochemistry)

Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

Daniel Barrera¹, Sierra Upton², Megan Rauch³, Tara Notarianni⁴, Ki Suk Eum⁵, Megan Liberty⁶, Sarmila Venkoba Sah⁷, Robert Liu⁸, Sean Newcomer⁹, Linda E. May¹⁰, Emre Agbas¹¹, Jessica Sage¹², Edina Kosa⁷, Abdulbaki Agbas^7,13

¹AdventHelath, ²University of Illinois Chicago School of Public Health, ³Lincoln Memorial University, ⁴Children’s Mercy Hospital, ⁵Scripps Clinic, ⁶Cincinnati Children’s Hospital Medical Center, ⁷Kansas City University, ⁸Roblex Rex Veterans Affairs Medical Center, ⁹California State University San Marcos, ¹⁰East Carolina University, ¹¹Massachusetts Institute of Technology, ¹²Boehringer Ingelheim Norway KS, ¹³Heartland Center for Mitochondrial Medicine

Journal (Biochemistry)

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Danye Qiu¹, Verena B. Eisenbeis¹, Adolfo Saiardi², Henning J. Jessen¹

¹Institute of Organic Chemistry,University of Freiburg, ²Medical Research Council, Laboratory for Molecular Cell Biology,University College London

Journal (Biochemistry)

Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification

William D. McCaig¹, Matthew A. Deragon¹, Phillip V. Truong¹, Angeleigh R. Knapp¹, Timothy J. LaRocca¹

¹Department of Basic and Clinical Sciences,Albany College of Pharmacy and Health Sciences

Journal (Biochemistry)

Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription

Phillip Wibisono¹, Jingru Sun¹

¹Department of Translational Medicine and Physiology, Elson S. Floyd College of Medicine,Washington State University

Journal (Biochemistry)

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Heidi L. Fresenius¹, Matthew L. Wohlever¹

¹Department of Chemistry & Biochemistry,University of Toledo

Journal (Biochemistry)

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Natalia Kopik^1,2, Olga Chrobak^1,2, Przemyslaw Latoch^1,3, Mariia Kovalenko^1,2, Agata L. Starosta^1,2

¹Laboratory of Gene Expression, ECOTECH-Complex,Maria Curie-Sklodowska University, ²Department of Molecular Biology, Institute of Biological Sciences,Maria Curie-Sklodowska University, ³Faculty of Information Technology,Polish-Japanese Academy of Information Technology

Journal (Biochemistry)

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Claudine Bisson^1,2, Corey W. Hecksel^3,4, James B. Gilchrist³, M. Alejandra Carbajal¹, Roland A. Fleck¹

¹Centre for Ultrastructural Imaging,New Hunt’s House, Guy’s Campus, King’s College London, ²Department of Biological Science,Birkbeck College, University of London, ³Electron Bio-Imaging Centre,Diamond Light Source, Harwell Science and Innovation Campus, ⁴SLAC National Accelerator Laboratory,Stanford University

Journal (Biochemistry)

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Emmanuel L. Crespo^1,2, Andreas Bjorefeldt¹, Mansi Prakash¹, Ute Hochgeschwender^1,2,3

¹College of Medicine,Central Michigan University, ²Biochemistry, Cell and Molecular Biology Program,Central Michigan University, ³Neuroscience Program,Central Michigan University

Journal (Biochemistry)

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Laura K. Hillert-Richter¹, Inna N. Lavrik¹

¹Translational Inflammation Research, Center of Dynamic Systems,Otto von Guericke University Magdeburg

Journal (Biochemistry)

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Mustafa Bulut¹, Alisdair R. Fernie^1,2, Saleh Alseekh^1,2

¹Max-Planck-Institute of Molecular Plant Physiology, ²Center of Plant Systems Biology and Biotechnology

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